Human IFN-alpha 6/IFNA6 Antibody

Catalog #: MAB10212 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10212-100
MAB10212-SP

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Detection of Human IFN-alpha 6/IFNA6 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IFN-alpha 6/IFNA6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IFN-alpha 6/IFNA6 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 1009720

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IFN-alpha 6/IFNA6
Leu28-Lys207
Accession # Q90W0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IFN-alpha 6/IFNA6 by Western Blot. Western blot shows lysates of HEK001 human epidermal keratinocyte cell line and NHEK human normal epidermal keratinocytes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IFN-alpha 6/IFNA6 Monoclonal Antibody (Catalog # MAB10212) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IFN-alpha 6/IFNA6 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha 6/IFNA6

Interferon kappa, also known as IFN-kappa, is a 207 aminoacids protein that in humans is encoded by the IFNK gene. IFN-kappa is a member of the type I interferon family which also includes IFN-alpha, -beta, -epsilon, and -omega. Type I interferons are a group of related glycoproteins that play an important role in host defenses against viral infections. Human IFN-kappa has been detected in keratinocytes, monocytes, and monocyte-derived dendritic cells and is reported to have contact-dependent antiviral activity. Human papillomavirus (HPV) 16 oncogene expression, which is necessary for the development of cervical cancer, has been shown to down-regulate human IFN-kappa expression.

Long Name

Interferon alpha 6

Entrez Gene IDs

3443 (Human); 15969 (Mouse)

Alternate Names

IFNA6; IFN-alpha 6; IFNalpha K; IFN-alpha K; IFN-kappa; interferon kappa; interferon, kappa; RP11-27J8.1; UNQ6124/PRO20084