Human IL-28A/IFN-lambda 2 Antibody (248512)

R&D Systems | Catalog # MAB15871

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

ELISA Capture (Matched Antibody Pair)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 248512
View all IL-28A/IFN-lambda 2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL‑28A/IFN‑ lambda 2
Val26-Val200
Accession # Q8IZJ0

Specificity

Detects human IL‑28A/IFN‑ lambda 2 in ELISAs. In sandwich immunoassays, 3.5% cross-reactivity with human IL-28B/IFN-lambda 3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human IL-28A/IFN-lambda 2 Antibody (248512)

Human IL-28A / IFN-lambda 2 ELISA Standard Curve

Human IL-28A / IFN-lambda 2 ELISA Standard Curve

Recombinant Human IL‑28A/IFN‑ lambda 2 (Catalog # 1587-IL) was serially diluted and captured by Mouse Anti-Human IL‑28A/IFN‑ lambda 2 Monoclonal Antibody (Catalog # MAB15871) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑28A/IFN‑ lambda 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1587) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human IL-28A/IFN-lambda 2 Antibody (248512)

Human IL-28A/IFN-lambda 2 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human IL‑28A/IFN-lambda 2 Biotinylated Antibody (Catalog # BAF1587)
  • Standard: Recombinant Human IL-28A/IFN-lambda 2 Protein (Catalog # 1587-IL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB15871 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-28A/IFN-lambda 2

IL-28A, IL-28B, and IL-29, also named interferon-lambda 2 (IFN-lambda 2), IFN-lambda 3, and IFN-lambda 1, respectively, are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11-13% aa sequence identity) and type I IFN family (15-19% aa sequence identity) (1-3). The genes encoding these three cytokines are localized to chromosome 19 and each is composed of multiple exons. The exon organization of these genes is also found in the IL-10 family genes but is distinct from the type I IFNs, which are encoded within a single exon, The expression of IL-28A, B, and IL-29 is induced by virus infection or double-stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor  beta (IL-10 R beta ) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation. The phosphorylated STAT1 and STAT2 complex with IFN-regulatory factor 9 (IRF-9) to form the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex that is translocated to the nucleus. ISGF-3 binds to the IFN-stimulated response element (ISRE) present in the regulatory regions of the target genes. Human IL-28A cDNA encodes a 200 amino acid (aa) residue precursor protein with a putative 25 aa signal peptide. It shares 94% and 67% aa sequence identity with human IL-28B and human IL-29, respectively.

References

  1. Vilcek, J. (2003) Nature Immunol. 4:8.
  2. Sheppard, P. et al. (2003) Nature Immunol. 4:63.
  3. Kotenko, S.V. et al. (2003) Nature Immunol. 4:69.

Long Name

Interleukin 28A

Alternate Names

IFN-lambda 2, IL28A

Entrez Gene IDs

282616 (Human)

Gene Symbol

IFNL2

UniProt

Q8IZJ0

Additional IL-28A/IFN-lambda 2 Products

Product Documents for Human IL-28A/IFN-lambda 2 Antibody (248512)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL-28A/IFN-lambda 2 Antibody (248512)

For research use only

Related Research Areas

Interferons

Customer Reviews for Human IL-28A/IFN-lambda 2 Antibody (248512) (1)

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  • Human IL-28A/IFN-lambda 2 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/13/2019
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human IL-28A/IFN-lambda 2 Antibody (248512) MAB15871

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Associated Pathways

Toll-Like Receptor Signaling Pathways
Toll-Like Receptor Signaling Pathway Thumbnail