Human Importin alpha 3/KPNA4 Antibody (843124)

R&D Systems | Catalog # MAB8204

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 843124
View all Importin alpha 3/KPNA4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Importin alpha 3/KPNA4
Met1-Asn104
Accession # O00629

Specificity

Detects human Importin alpha 3/KPNA4 in ELISA and Western blot. In direct ELISA, no cross-reactivity to human KPNA3 was detected.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Importin alpha 3/KPNA4 Antibody (843124)

Detection of Human Importin a3/KPNA4 antibody by Western Blot.

Detection of Human Importin alpha 3/KPNA4 by Western Blot.

Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Importin a3/KPNA4 Monoclonal Antibody (Catalog # MAB8204) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Importin a3/KPNA4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Importin a3/KPNA4 antibody by Simple WesternTM.

Detection of Human Importin alpha 3/KPNA4 by Simple WesternTM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Importin alpha 3/KPNA4 at approximately 56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Importin alpha 3/KPNA4 Monoclonal Antibody (Catalog # MAB8204). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human Importin alpha 3/KPNA4 Antibody (843124)

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: NTera‑2 human testicular embryonic carcinoma cell line and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Importin alpha 3/KPNA4

KPNA4 (Karyopherin subunit alpha 4), also called Importin subunit a4 or Importin aQ1 (previously called Qip1 or Importin a3) is an approximately 58-60 kDa member of the Importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. KPNA4 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus.  Human KPNA4 is a 521 amino acid (aa) protein that contains an N-terminal IBB/Importin b domain (aa 2-58), ten Armadillo repeats that bind “cargo” (aa 66-485) and two intervening NLS binding sites. Human KPNA4 aa 1-104 share 99% aa identity with mouse and rat KPNA4, which in turn share 100% identity with each other.

Long Name

Karyopherin alpha 4

Alternate Names

KPNA4, QIP12, SRP3

Entrez Gene IDs

3840 (Human); 16649 (Mouse); 361959 (Rat)

Gene Symbol

KPNA4

UniProt

O00629

Additional Importin alpha 3/KPNA4 Products

Product Documents for Human Importin alpha 3/KPNA4 Antibody (843124)

Certificate of Analysis

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Product Specific Notices for Human Importin alpha 3/KPNA4 Antibody (843124)

For research use only

Related Research Areas

Cell Cycle

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