Human Jade-1/PHF17 Alexa Fluor® 647-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Jade-1/PHF17

Jade-1 (Gene for Apoptosis and Differentiation in Epithelia; also PHF17) is a 94 kDa member of the JADE family of proteins. It is expressed in renal tubule cells as well as embryonic primitive streak (in mouse). Initially, Jade-1 was described as a proapoptotic tumor suppressor. Jade-1 also interacts with the HBO1 complex to specifically acetylate nucleosome histones, thus acting as a transcriptional activator that likely impacts DNA replication, recombination and repair. Human Jade-1 is 842 amino acids (aa) in length. It contains one PEST domain with multiple Ser phosphorylation sites (aa 5-28), and two PHD Zinc fingers (aa 203-253 and 312-371) that are required for histone acetylation. There are two splice variants, one that is 61-64 kDa in size and contains a 7 aa substitution for aa 503-842, and another that shows a deletion of aa 99-110. Over aa 1-137, human Jade-1 shares 96% aa identity with mouse Jade-1.

Product Datasheets

Related Research Areas

• Epigenetics
• Stem Cell Epigenetics