Human LIF Quantikine ELISA Kit

R&D Systems | Catalog # DLF00B

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)

Sensitivity

5.36 pg/mL

Assay Range

31.3-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Human LIF Quantikine ELISA Kit

The Quantikine® Human LIF Immunoassay is a 4.5 hours solid phase ELISA designed to measure human LIF in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant human LIF and antibodies raised against the recombinant protein. Results obtained for naturally occurring human LIF showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human LIF.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human LIF.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

精度

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 255 778 1433 268 827 1548
Standard Deviation 11.0 21.8 32.6 21.0 50.9 76.7
CV% 4.3 2.8 2.3 7.8 6.2 5.0

Recovery for Human LIF Quantikine ELISA Kit

The recovery of human LIF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 92-112
EDTA Plasma (n=4) 90 83-96
Heparin Plasma (n=4) 90 83-99
Serum (n=4) 92 83-101

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human LIF were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. 

Human LIF ELISA Linearity

Scientific Data Images for Human LIF Quantikine ELISA Kit

Human LIF ELISA Cell Culture Supernate Standard Curve

Human LIF ELISA Cell Culture Supernate Standard Curve

Human LIF ELISA Standard Curve

Human LIF ELISA Standard Curve

Human LIF ELISA Serum/Plasma Standard Curve

Human LIF ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LIF

LIF (Leukemia Inhibitory Factor) is a pleiotropic member of the IL-6 family of cytokines. It functions through a heterodimeric receptor complex containing a ligand-binding subunit, LIF R alpha/CD118, and a signal transducing subunit, gp130. gp130 also serves as a subunit of the receptor complexes for Oncostatin M, Cardiotrophin-1, CNTF, IL-6, IL-11, and IL-27. LIF supports uterine implantation of the embryo, pluripotency in embryonic stem cells, and proliferation of progenitor cells. It promotes regulatory T cell and inhibits Th17 cell differentiation, and it is required by the thymic epithelium to support T cell maturation. It promotes motor neuron survival and oligodendrocyte myelination, enhances adrenal production of cortisol and aldosterone, and promotes differentiation of adipocytes and cardiac smooth muscle cells. LIF can also function as an autocrine growth factor in pancreatic cancer and can induce formation of immunosuppressive tumor-associated macrophages.

Long Name

Leukemia Inhibitory Factor

Alternate Names

D Factor, Emfilermin, HILDA, MLPLI

Entrez Gene IDs

3976 (Human); 16878 (Mouse); 403449 (Canine)

Gene Symbol

LIF

Additional LIF Products

Product Documents for Human LIF Quantikine ELISA Kit

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human LIF Quantikine ELISA Kit

For research use only

Citations for Human LIF Quantikine ELISA Kit

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