Human LILRA5/CD85f Antibody (711827)

R&D Systems | Catalog # MAB67541

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 711827
View all LILRA5/CD85f Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human LILRA5/CD85f
Gly42-Arg268
Accession # A6NI73

Specificity

Detects human LILRA5/CD85f in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse LILRC1, recombinant rat LILRC1, recombinant human (rh) ILT7/LILRA4, rhILT4, rhILT5, or rhLILRB4/LIR5 is observed. In Western blots,no cross‑reactivity with rhILT7/LILRA4 or rhILT5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human LILRA5/CD85f Antibody (711827)

Detection of Human LILRA5/CD85f antibody by Western Blot.

Detection of Human LILRA5/CD85f by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LILRA5/CD85f Monoclonal Antibody (Catalog # MAB67541) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ILT11/LILRA5 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human LILRA5/CD85f Antibody (711827)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LILRA5/CD85f

ILT11 (also leukocyte immunoglobulin-like receptor 9 (LIR-9), CD85f, and LILRA5) is a 39-40 kDa Group 2 member of the LILR family of innate immune receptors. It is expressed on monocytes and perhaps neutrophils, and its activation results in the secretion of proinflammatory cytokines such as TNF-alpha and IL-1 beta. Mature human ILT11 is a 258 amino acid (aa) type I transmembrane glycoprotein.  It has a 227 aa extracellular domain (aa 42-268) that contains two C2-type Ig-like domains (aa 51‑136 and 142-230), and a 10 aa cytoplasmic tail. ILT11 has three potential splice forms. One is a 35 kDa soluble form of the molecule described above that shows a 27 aa substitution for aa 239-299. The other two splice forms are analogous to the above membrane and soluble forms, but demonstrate signal sequence cleavage further downstream after Ala51. Over aa 41-268, human ILT11 shares 58% aa identity with mouse ILT11.

Long Name

Leukocyte Immunoglobulin-like Receptor Subfamily A Member 5

Alternate Names

CD85f, ILT11, LILRB7, LIR9

Entrez Gene IDs

353514 (Human); 232801 (Mouse); 691533 (Rat)

Gene Symbol

LILRA5

UniProt

A6NI73 (Human)

Additional LILRA5/CD85f Products

Product Documents for Human LILRA5/CD85f Antibody (711827)

Certificate of Analysis

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Product Specific Notices for Human LILRA5/CD85f Antibody (711827)

For research use only

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