Human LILRA5/CD85f Antibody

Catalog #: MAB67541 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB67541
MAB67541-SP

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Detection of Human LILRA5/CD85f by Western Blot.

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Human LILRA5/CD85f Antibody Summary

Species Reactivity

Human

Specificity

Detects human LILRA5/CD85f in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse LILRC1, recombinant rat LILRC1, recombinant human (rh) ILT7/LILRA4, rhILT4, rhILT5, or rhLILRB4/LIR5 is observed. In Western blots,
no cross‑reactivity with rhILT7/LILRA4 or rhILT5 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 711827

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human LILRA5/CD85f
Gly42-Arg268
Accession # A6NI73

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human LILRA5/CD85f by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LILRA5/CD85f Monoclonal Antibody (Catalog # MAB67541) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ILT11/LILRA5 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRA5/CD85f

ILT11 (also leukocyte immunoglobulin-like receptor 9 (LIR-9), CD85f, and LILRA5) is a 39-40 kDa Group 2 member of the LILR family of innate immune receptors. It is expressed on monocytes and perhaps neutrophils, and its activation results in the secretion of proinflammatory cytokines such as TNF-alpha and IL-1 beta. Mature human ILT11 is a 258 amino acid (aa) type I transmembrane glycoprotein. It has a 227 aa extracellular domain (aa 42-268) that contains two C2-type Ig-like domains (aa 51‑136 and 142-230), and a 10 aa cytoplasmic tail. ILT11 has three potential splice forms. One is a 35 kDa soluble form of the molecule described above that shows a 27 aa substitution for aa 239-299. The other two splice forms are analogous to the above membrane and soluble forms, but demonstrate signal sequence cleavage further downstream after Ala51. Over aa 41-268, human ILT11 shares 58% aa identity with mouse ILT11.

Long Name

Leukocyte Immunoglobulin-like Receptor Subfamily A Member 5

Entrez Gene IDs

353514 (Human); 232801 (Mouse); 691533 (Rat)

Alternate Names

CD85 antigen-like family member F; CD85; CD85f antigen; CD85f; ILT11; ILT-11; ILT11CD85f; Immunoglobulin-like transcript 11; leukocyte Ig-like receptor 9; Leukocyte immunoglobulin-like receptor 9; leukocyte immunoglobulin-like receptor subfamily A member 5 soluble; leukocyte immunoglobulin-like receptor subfamily A member 5; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRA5; LILRB7; LIR9; LIR-9; LIR9immunoglobulin-like transcript 11 protein; member 7