Human LMP2/PSMB9 Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB7709G Datasheet / COA / SDS

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FAB7709G-100UG

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All LMP2/PSMB9 Antibodies

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Human LMP2/PSMB9 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human LMP2/PSMB9 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human PSMB6 is observed.

Source

Monoclonal Mouse IgG2b Clone # 792520

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human LMP2/PSMB9
Thr21-Glu219
Accession # P28065

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: LMP2/PSMB9

LMP2 (large multifunctional peptidase 2), also called PSMB9 (proteasome subunit beta 9) or PSMB6i, is a 22 kDa broad-specificity aminopeptidase of the T1B family that is a component of the 20S immunoproteasome core (a7b7b7a7) of the 26S proteasome particle. Induction of LMP2 by IFN-g, IRF-1, TNF-a, CD40L, or heat shock in immune-responsive cells or cancer cells promotes replacement of proteasome subunit PSMB6 by LMP2 and processing of peptides for presentation by MHC I. After auto-cleavage between amino acids (aa) 20-21, human LMP2 (aa 21-219) shares 91% aa sequence identity with mouse and rat LMP2. Alternate splicing creates a short form, LMP2.S, which lacks aa 4-13 within the propeptide.

Long Name

Proteasome (Prosome, Macropain) Subunit, beta Type, 9

Entrez Gene IDs

5698 (Human); 16912 (Mouse); 24967 (Rat)

Alternate Names

beta1i; LMP2; LMP2MGC70470; Low molecular mass protein 2; Macropain chain 7; Multicatalytic endopeptidase complex chain 7; proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctionalpeptidase 2); proteasome catalytic subunit 1i; Proteasome chain 7; proteasome subunit beta 6i; proteasome subunit beta type-9; Proteasome subunit beta-1i; proteasome-related gene 2; PSMB6iproteasome (prosome, macropain) subunit, beta type, 9 (large multifunctionalprotease 2); PSMB9; Really interesting new gene 12 protein; RING12; RING12EC 3.4.25.1