Human Lysine (K)-specific Demethylase 6B/KDM6B Alexa Fluor® 647-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Lysine (K)-specific Demethylase 6B/KDM6B

KDM6B (Lysine [K]-specific demethylase 6B; also JMJD3) is a 170-185 kDa member of the UTX family of enzymes. It is expressed in both embryo and adult, and specifically demethylates tri- and di-methylated Lys27 on histone H3 (H3K27). Methylation of H3K27 by PRC2 inactivates genes, while demethylation of methylated H3K27 by KDM6B activates genes. Human KDM6B is 1643 amino acids (aa) in length. It contains two Pro-rich regions (aa 32-85 and 1046-1082) plus one jumonji domain (aa 1339-1502). One potential splice variant is reported that contains a 39 aa insertion after Leu1636. Over aa 1530-1682, which is a sequence that contains the 39 aa insertion, human KDM6B shares 75% aa sequence identity with mouse KDM6B. When the insertion is disregarded, human and mouse KDM6B are identical in aa sequence over the above range of amino acids.

Product Datasheets

Related Research Areas

• Epigenetics
• Methyltransferases and Demethylases
• Stem Cell Epigenetics