Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 101721
View all MMP-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMP‑2
Ala30-Cys660
Accession # P08253.2

Specificity

Detects pro and active forms of human MMP-2. In direct ELISAs, 100% cross-reactivity with recombinant mouse MMP-2 is observed. In dot blots, no cross-reactivity with recombinant human MMP-1, -3, -7, -8, -9, -10, -12, or -13 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human MMP-2 Antibody (101721)

Detection of MMP-2 by Western Blot

Detection of MMP-2 by Western Blot

Expression and activity assays of MMP2 and MMP9 in two cell lines (HT-29 and HT-1080). (A) Gel electrophoresis and (B) real-time PCR showing mRNA expressions of proMMP2 and proMMP9 relative to that of GAPDH in HT-29 and HT-1080. The asterisk denotes significant difference between HT-29 and HT-1080 (* p < 0.05, *** p < 0.001, Student’s t test, n = 3). (C) Western blot and (D) gelatin zymographic images of (pro)MMP2 and (pro)MMP9 in the CM of HT-29 or HT-1080. Active MMP2 or MMP9 enzyme was loaded into the first lane. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35163529), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human MMP-2 Antibody (101721)

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑2 (Catalog # 902-MP), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human MMP‑2 Western Blot Standard (Catalog # WBC025)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-2

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP‑2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP‑1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP‑2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP‑2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting; and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites.

Long Name

Matrix Metalloproteinase 2

Alternate Names

Gelatinase A, MMP2

Entrez Gene IDs

4313 (Human); 17390 (Mouse)

Gene Symbol

MMP2

UniProt

P08253.2

Additional MMP-2 Products

Product Documents for Human MMP-2 Antibody (101721)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MMP-2 Antibody (101721)

For research use only

Citations for Human MMP-2 Antibody (101721)

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