Human MMP-9 Antibody (526501)

R&D Systems | Catalog # MAB11756

R&D Systems
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 526501
View all MMP-9 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMP‑9
Ala20-Asp707 (Gln279Arg)
Accession # P14780

Specificity

Detects recombinant mouse MMP-9 protein in Direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for Human MMP-9 Antibody (526501)

Detection of Human MMP‑9 by Western Blot.

Western Blot shows lysates of human spleen tissue. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human MMP‑9 Monoclonal Antibody (Catalog # MAB11756) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP‑9 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human MMP‑9 by Simple WesternTM.

Left: Simple Western lane view shows lysates of human spleen, loaded at 0.5 mg/ml. A specific band was detected for MMP‑9 at approximately 150 kDa (as indicated) using both 10 µg/ml and 50 µg/ml of Mouse Anti-Human MMP‑9 Monoclonal Antibody (Catalog # MAB11756) followed by HRP-conjugated Goat Anti-Mouse Secondary Antibody (Catalog # 042-205). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Right: Simple Western electropherogram showing the same Mouse Anti-Human MMP‑9 Monoclonal Antibody (Catalog # MAB11756) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the human spleen.

Applications for Human MMP-9 Antibody (526501)

Application
Recommended Usage

Simple Western

10-50 µg/mL
Sample: Human spleen

Western Blot

2 µg/mL
Sample: Human spleen

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-9

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (Gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain.

Long Name

Matrix Metalloproteinase 9

Alternate Names

CLG4B, Gelatinase B, GELB, MANDP2, MMP9

Entrez Gene IDs

4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)

Gene Symbol

MMP9

UniProt

P14780

Additional MMP-9 Products

Product Documents for Human MMP-9 Antibody (526501)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human MMP-9 Antibody (526501)

For research use only

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