Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Histone H4 Primary Antibodies »

Product Specifications

Immunogen

Tetrahymena pyriformis acetylated Histone H4 synthetic peptide
AGGKGGKGMGKVGAKRHSK
Accession # P02310

Specificity

Detects immunogen in direct ELISAs and human Acetyl Histone H4 in Western blots. Reactivity with non-acetylated peptide was removed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse Acetyl Histone H4 Antibody

Detection of Human Acetyl Histone H4 antibody by Western Blot.

Detection of Human Acetyl Histone H4 by Western Blot.

Western blot shows lysates of Tera-2 human embryonic lung carcinoma cell line, Nalm-6 human Pre-B acute lymphocytic leukemia cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Acetyl Histone H4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5215) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Acetyl Histone H4 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Histone H4 antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC).

Histone H4 in NTera‑2 Human Cell Line.

Histone H4 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Sheep Anti-Human Acetyl Histone H4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5215) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse Acetyl Histone H4 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed NTera‑2 human testicular embryonic carcinoma cell line

Western Blot

1 µg/mL
Sample: Tera‑2 human embryonic lung carcinoma cell line, Nalm‑6 human Pre-B acute lymphocytic leukemia cell line, and Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Histone H4

Histone 4 (H4/HIST1H4I; also H4M and HIST4H4) is a 12-15 kDa member of the histone H4 family of molecules. It is ubiquitously expressed, with H4 initially synthesized and deposited in replicating chromatin during S phase. H4 undergoes multiple posttranslational modifications. At the G2/M transition, H4 is monomethylated on Lys20 and possibly contributes to transcription initiation/elongation. As the cell moves into the M/G1 phase, Lys20 becomes either dimethylated or trimethylated, contributing to DNA repair and telomere maintenance, respectively. H4 is also known to be phosphorylated on Ser2 and 48, plus Tyr52 and 89, and acetylated on at least nine sites. Human histone 4 is 103 amino acids (aa) in length. Full length histone 4 is identical in aa sequence to mouse histone 4, and 78% aa identical to Tetrahymena histone 4 over aa 2-20 used for immunization.

Alternate Names

H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4

Entrez Gene IDs

121504 (Human); 319159 (Mouse)

Gene Symbol

H4-16

UniProt

P02310

Additional Histone H4 Products

Product Documents for Human/Mouse Acetyl Histone H4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Acetyl Histone H4 Antibody

For research use only

Related Research Areas

Epigenetics
Notch Pathway

Citations for Human/Mouse Acetyl Histone H4 Antibody

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Protocols

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