Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Dynactin Subunit 2/DCTN2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Dynactin Subunit 2/DCTN2
Asp277-Lys401
Accession # Q13561

Specificity

Detects human Dynactin Subunit 2/DCTN2 in direct ELISAs and human and mouse Dynactin Subunit 2/DCTN2 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human DCTN1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse Dynactin Subunit 2/DCTN2 Antibody

Detection of Human and Mouse Dynactin Subunit 2/DCTN2 antibody by Western Blot.

Detection of Human and Mouse Dynactin Subunit 2/DCTN2 by Western Blot.

Western blot shows lysates of human placenta tissue and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Dynactin Subunit 2/DCTN2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6850) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Dynactin Subunit 2/ DCTN2 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Dynactin Subunit 2/DCTN2 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Dynactin Subunit 2/DCTN2 in HeLa Human Cell Line.

Dynactin Subunit 2/DCTN2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Dynactin Subunit 2/DCTN2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6850) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse Dynactin Subunit 2/DCTN2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed unstimulated HeLa human cervical epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: Human placenta tissue and CTLL‑2 mouse cytotoxic T cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dynactin Subunit 2/DCTN2

DCTN-2 (Dynactin-2; also 50 kDa dynein-associated polypeptide and p50 dynamitin) is a 50 kDa intracellular member of the dynactin subunit 2 family of phosphoproteins. It is ubiquitously expressed and serves to stabilize the dynactin complex of 11 different proteins. This complex is an obligate cofactor of dynein and kinesin motors, which position the mitotic spindle for cell division, and move vesicles within the cell. Human DCTN-2 is 401 amino acids (aa) in length. It contains three coiled‑coil regions (aa 99-132, 214-244 and 379-399), two potential phosphorylation sites (Ser83 and Tyr86), and one acetylation site at Ala2. The first two coiled‑coil regions contribute to oligomerization, while aa 1-91 are essential for dynactin regulation. Potential isoform variants utilize either a five or two aa insertion after Ala35, an alternative start site at Met88, and a 21 aa substitution for aa 118-401. Over aa 277-401, human DCTN-2 shares 95% aa identity with mouse DCTN-2.

Alternate Names

DCTN2, Dynamitin, RBP50

Entrez Gene IDs

10540 (Human); 69654 (Mouse); 299850 (Rat)

Gene Symbol

DCTN2

UniProt

Q13561 (Human)

Additional Dynactin Subunit 2/DCTN2 Products

Product Documents for Human/Mouse Dynactin Subunit 2/DCTN2 Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse Dynactin Subunit 2/DCTN2 Antibody

For research use only

Related Research Areas

Microtubules

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Protocols

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