GDPD2 Antibody (501016)

R&D Systems | Catalog # MAB7026

R&D Systems
Discontinued Product
MAB7026 has been discontinued. View all GDPD2 products.

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 501016
View all GDPD2 Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human GDPD2
Accession # Q9HCC8

Specificity

Detects human and mouse GDPD2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for GDPD2 Antibody (501016)

GDPD2 antibody in MC3T3-E1 Mouse Cell Line by Immunocytochemistry (ICC).

GDPD2 in MC3T3‑E1 Mouse Cell Line.

GDPD2 was detected in immersion fixed MC3T3-E1 mouse preosteoblast cell line using Mouse Anti-Human GDPD2 Monoclonal Antibody (Catalog # MAB7026) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for GDPD2 Antibody (501016)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MC3T3‑E1 mouse preosteoblast cell line

Reviewed Applications

Read 1 review rated 5 using MAB7026 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GDPD2

Glycerophosphodiester phosphodiesterase 3 (GDPD2), also known as GDE3, is a 65 kDa 7TM cell surface protein that is highly expressed in bone and spleen. GDPD2 promotes osteoblast differentiation and induces changes in the actin cytoskeleton. Its 225 amino acid (aa) N-terminal ECD contains a domain that is homologous to bacterial glycerophosphodiester phosphodiesterases. Human GDPD2 shares 85% aa sequence identity with mouse and rat GDPD2.

Long Name

Glycerophosphodiester Phosphodiesterase Domain Containing 2

Alternate Names

GDE3, OBDPF

Entrez Gene IDs

54857 (Human); 71584 (Mouse); 302421 (Rat)

Gene Symbol

GDPD2

UniProt

Q9HCC8

Additional GDPD2 Products

Product Documents for GDPD2 Antibody (501016)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for GDPD2 Antibody (501016)

For research use only

Customer Reviews for GDPD2 Antibody (501016) (1)

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  • Human/Mouse GDPD2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: MC3T3-E1 mouse preosteoblast cell line
    Species: Mouse
    Verified Customer | Posted 05/20/2022
    GDPD2 Antibody (501016) MAB7026

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