Human/Mouse GDPD2 Antibody

Name: Human/Mouse GDPD2 Antibody
Brand: R&D Systems
SKU: MAB7026
Rating: 5 (1 reviews)

Catalog #: MAB7026 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7026
MAB7026-SP

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Human/Mouse GDPD2 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse GDPD2.

Source

Monoclonal Mouse IgG_2A Clone # 501016

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human GDPD2
Accession # Q9HCC8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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GDPD2 in MC3T3‑E1 Mouse Cell Line. GDPD2 was detected in immersion fixed MC3T3-E1 mouse preosteoblast cell line using Mouse Anti-Human GDPD2 Monoclonal Antibody (Catalog # MAB7026) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDPD2

Glycerophosphodiester phosphodiesterase 3 (GDPD2), also known as GDE3, is a 65 kDa 7TM cell surface protein that is highly expressed in bone and spleen. GDPD2 promotes osteoblast differentiation and induces changes in the actin cytoskeleton. Its 225 amino acid (aa) N-terminal ECD contains a domain that is homologous to bacterial glycerophosphodiester phosphodiesterases. Human GDPD2 shares 85% aa sequence identity with mouse and rat GDPD2.

Long Name

Glycerophosphodiester Phosphodiesterase Domain Containing 2

Entrez Gene IDs

54857 (Human); 71584 (Mouse); 302421 (Rat)

Alternate Names

EC 3.1.4.43; FLJ20207; GDE3; GDE3Osteoblast differentiation promoting factor; GDPD2; Glycerophosphodiester phosphodiesterase 3; glycerophosphodiester phosphodiesterase domain containing 2; Glycerophosphodiester phosphodiesterase domain-containing protein 2; OBDPF; OBDPFglycerophosphoinositol inositolphosphodiesterase GDPD2