Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Haptoglobin Primary Antibodies »

Product Specifications

Immunogen

Human Haptoglobin from human serum

Specificity

Detects human and mouse Haptoglobin in Western blots. In sandwich immunoassays, human Haptoglobin is detected.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse Haptoglobin Antibody

Detection of Human and Mouse Haptoglobin antibody by Western Blot.

Detection of Human and Mouse Haptoglobin by Western Blot.

Western blot shows human plasma. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Haptoglobin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8465) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Haptoglobin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human and Mouse Haptoglobin antibody by Western Blot.

Detection of Human and Mouse Haptoglobin by Western Blot.

Western blot shows lysates of human Haptoglobin purified from human serum and mouse haptoglobin purified from mouse serum. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse Haptoglobin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8465) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Haptoglobin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Haptoglobin antibody by Simple WesternTM.

Detection of Human Haptoglobin by Simple WesternTM.

Simple Western lane view shows human serum, loaded at 1:25000 dilution. A specific band was detected for Haptoglobin at approximately 60 kDa (as indicated) using 1 µg/mL of Goat Anti-Human/Mouse Haptoglobin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8465) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Human Haptoglobin Antibody in ELISA Standard Curve.

Human Haptoglobin ELISA Standard Curve.

Recombinant Human Haptoglobin protein was serially diluted 2-fold and captured by Mouse Anti-Human Haptoglobin Monoclonal Antibody (Catalog # MAB8465) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Haptoglobin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8465) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human/Mouse Haptoglobin Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Haptoglobin Monoclonal Antibody (Catalog # MAB8465).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Haptoglobin DuoSet ELISA Kit (Catalog # DY8465-05) for convenient development of a sandwich ELISA or the Human Haptoglobin Quantikine ELISA Kit (Catalog # DHAPG0) for a complete optimized ELISA.

Simple Western

1 µg/mL
Sample: Human Serum

Western Blot

0.2 µg/mL
Sample: Human Plasma, Human Haptoglobin purified from human serum, and Mouse haptoglobin purified from mouse serum

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Haptoglobin

Haptoglobin is a 406 aminoacids (aa) protein that in humans is encoded by the HP gene. This gene encodes a preproprotein that is processed into alpha (aa 19-160) and beta (aa 162-406) chains that combine as a tetramer to produce haptoglobin. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage.

Alternate Names

HP, HP-1, hp2-alpha, HPA1S

Entrez Gene IDs

3240 (Human); 15439 (Mouse); 24464 (Rat)

Gene Symbol

HP

Additional Haptoglobin Products

Product Documents for Human/Mouse Haptoglobin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Haptoglobin Antibody

For research use only

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