Human/Mouse HMGA2 Alexa Fluor® 405-conjugated Antibody

Catalog #: AF3184V Datasheet / COA / SDS

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AF3184V-100UG

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Human/Mouse HMGA2 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse HMGA2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human HMGB1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human HMGA2
Ser2-Asp109
Accession # P52926

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: HMGA2

HMGA2, previously known as HMGI-C, belongs to the group of high mobility chromosomal proteins that have the AT-hook DNA-binding motif. It is highly and ubiquitously expressed during embryogenesis. Chromosomal rearrangements of the HMGA2 gene and overexpression are frequently associated with benign and malignant tumors, respectively. Human and mouse HMGA2 share 95% amino acid sequence identity.

Long Name

High Mobility Group AT-hook 2

Entrez Gene IDs

8091 (Human)

Alternate Names

BABL; BABLHMGI-C; high mobility group AT-hook 2; High mobility group AT-hook protein 2; high mobility group protein HMGI-C; high-mobility group (nonhistone chromosomal) protein isoform I-C; High-mobility group protein HMGI-C; HMGA2; HMGIC; HMGICSTQTL9; LIPO