Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ID1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse ID1
Met1-Glu135
Accession # P20067

Specificity

Detects human and mouse ID1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse ID1 Antibody

Detection of Human ID1 antibody by Western Blot.

Detection of Human ID1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MCF-7 human breast cancer cell line. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band for ID1 was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ID1 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

ID1 in BG01V Human Embryonic Stem Cells.

ID1 was detected in immersion fixed BG01V human embryonic stem cells, undifferentiated (lower panel) and differentiated into neural progenitor cells (upper panel), using Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4377) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human ID1 by Simple WesternTM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ID1 at approximately 30 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4377). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of ID1 in PC‑3 cells (Positive) & Daudi cells (Negative).

ID1 was detected in immersion fixed PC‑3 human prostate cancer cells (Positive) & absent in Daudi human Burkitt's lymphoma cells (Negative) using Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4377) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to Nuclear and cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse ID1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells differentiated to neural progenitor cells, PC‑3 human prostate cancer cells (Positive) & absent in Daudi human Burkitt's lymphoma cells (Negative)

Simple Western

20 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: ID1

ID-1a is a negative regulator of helix-loop-helix (HLH) DNA binding proteins. ID-1a contains a HLH motif but no DNA binding motif, therefore, upon binding other HLH proteins, ID-1a acts a dominant negative regulator. ID-1a can be found in both cytoplasmic and nuclear cell fractions. Increased ID-1a expression is associated with cellular proliferation and cancer.

Long Name

Inhibitor of DNA Binding 1

Alternate Names

BHLHB24, bHLHb24DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, dJ857M17.1.2, dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helixprotein), ID, inhibitor of differentiation 1, Inhibitor of DNA binding 1, inhibitor of DNA binding 1, dominant negative helix-loop-helix protein

Entrez Gene IDs

3397 (Human); 15901 (Mouse); 25261 (Rat)

Gene Symbol

ID1

UniProt

P20067

Additional ID1 Products

Product Documents for Human/Mouse ID1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse ID1 Antibody

For research use only

Citations for Human/Mouse ID1 Antibody

Customer Reviews for Human/Mouse ID1 Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse ID1 Antibody and earn rewards!

Have you used Human/Mouse ID1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies