Akt2 Antibody (302514)

Name: Akt2 Antibody (302514)
Brand: R&D Systems
SKU: MAB2315
Price: 399 USD
Availability: InStock

R&D Systems | Catalog # MAB2315

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₃ Clone # 302514

View all Akt2 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Akt2
Asn2-Glu481
Accession # P31751

Specificity

Detects human, mouse, and rat Akt2. Detects human Akt2 in direct ELISAs and endogenous Akt2 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Akt1 or rhAkt3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₃

Scientific Data Images for Akt2 Antibody (302514)

Detection of Human/Mouse/Rat Akt2 by Western Blot.

Western blot shows lysates of MDA-MB-468 human breast cancer cell line and Huh-7 human hepatoma cell line. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human/Mouse/Rat Akt2 Monoclonal Antibody (Catalog # MAB2315) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Active Akt1 (Catalog # 1775-KS), recombinant human Akt2, and recombinant human Akt3 (2 ng/lane) were included. A specific band for Akt2 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Akt2 Antibody (302514)

Application

Recommended Usage

Western Blot

2 µg/mL
Sample: MDA-MB-468 human breast cancer cell line and Huh-7 human hepatoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Akt2

The serine/threonine kinase Akt, also known as protein kinase B (PKB), is a central regulator of such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. In mammals, three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). Akt2 is expressed predominantly in insulin target tissues such as liver, skeletal muscle, and fat.