Human/Mouse/Rat B-Raf Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB3424G Datasheet / COA / SDS

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FAB3424G-100UG

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Human/Mouse/Rat B-Raf Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat B-Raf. No cross-reactivity with recombinant human Raf-1 is observed. Cross-reactivity with A-Raf has not been tested.

Source

Monoclonal Mouse IgG2a Clone # 336016

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human B-Raf
Met1-His766
Accession # P15056

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: B-Raf

The Raf serine/threonine kinases are effectors of Ras that function as MAP3Ks in the ERK phosphorylation cascade. Mammals express three Raf proteins: Raf-1 (C‑Raf); A-Raf; and B-Raf, strongly expressed in fetal brain and adult cerebrum. Mice with a targeted disruption of the B-raf gene die of vascular defects during mid‑gestation. B-raf mutations have been found in two-thirds of malignant melanomas, with the single substitution V599E in the kinase domain the most frequent occurrence.

Long Name

v-Raf Murine Sarcoma Viral Oncogene Homolog B1

Entrez Gene IDs

673 (Human); 114486 (Rat)

Alternate Names

94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRaf; B-Raf; BRAF1; B-RAF1; BRAF1MGC126806; EC 2.7.11.1; MGC138284; murine sarcoma viral (v-raf) oncogene homolog B1; NS7; p94; Proto-oncogene B-Raf; RAFB1; serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B1FLJ95109