Human/Mouse/Rat Casein Kinase 1 alpha Alexa Fluor® 647-conjugated Antibody

Catalog #: AF4569R Datasheet / COA / SDS

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AF4569R-100UG

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Human/Mouse/Rat Casein Kinase 1 alpha Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse, and rat CK1 alpha in Western blots. In Western blots, this antibody does not cross-react with recombinant human CK1δ or CK1 gamma ; reactivity with CK1 epsilon is unknown.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CK1Î±
Ala255-Phe365
Accession # NP_001020276

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Casein Kinase 1 alpha

Casein Kinase 1 (CK1) represents a group of serine/threonine protein kinases that are present in all eukaryotic organisms. Human CK1 isoforms ( alpha, gamma 1, gamma 2, gamma 3, δ, and epsilon ) act as monomeric constitutively active enzymes that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation, and circadian rhythms. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. CK1 alpha is speculated to play a role in mammalian cell cycle progression, spindle dynamics, and chromosome segregation.

Entrez Gene IDs

1452 (Human); 93687 (Mouse); 113927 (Rat)

Alternate Names

Casein Kinase 1 alpha; casein kinase 1, alpha 1; casein kinase I isoform alpha; CK1CKI-alpha; CSNK1A1; down-regulated in lung cancer; EC 2.7.11.1; HLCDGP1; PRO2975