FRS2 Antibody (462910)

R&D Systems | Catalog # MAB4069

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 462910
View all FRS2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FRS2
Asn121-Asn449
Accession # Q8WU20

Specificity

Detects human, mouse, and rat FRS2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for FRS2 Antibody (462910)

Detection of Human/Mouse/Rat FRS2 antibody by Western Blot.

Detection of Human/Mouse/Rat FRS2 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat FRS2 Monoclonal Antibody (Catalog # MAB4069) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FRS2 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for FRS2 Antibody (462910)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line

Reviewed Applications

Read 1 review rated 4 using MAB4069 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FRS2

FRS2 (FGF R substrate 2; also known as SNT and FRS2 alpha ) is a 70‑90 kDa member of the FRS family of lipid-anchored docking proteins. It is an intermediary between FGF R and other RTK receptors and their Ras/MAPK signaling cascades. FRS2 contains a membrane-anchoring myristoylation signal (aa 1‑6), a PTB domain (aa 13‑115) that interacts with FGF and NGF receptors, and a C-terminal Tyr-rich region (aa 196‑471) that serves as a docking site for GRB‑2 and SHP‑2.

Long Name

Fibroblast Growth Factor Receptor Substrate 2

Alternate Names

FRS2alpha, SNT1

Entrez Gene IDs

10818 (Human); 327826 (Mouse)

Gene Symbol

FRS2

UniProt

Q8WU20

Additional FRS2 Products

Product Documents for FRS2 Antibody (462910)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for FRS2 Antibody (462910)

For research use only

Related Research Areas

FGF Family
GDNF Family

Citations for FRS2 Antibody (462910)

Customer Reviews for FRS2 Antibody (462910) (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 19735729
    Species: Mouse
    Verified Customer | Posted 02/20/2015

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