HOMER1 Antibody (666241)

R&D Systems | Catalog # MAB6889

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 666241
View all HOMER1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide KFGQWADSRA corresponding to aa 73-82 of human HOMER1
Accession # Q86YM7

Specificity

Detects human HOMER1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for HOMER1 Antibody (666241)

Detection of Human HOMER1 antibody by Western Blot.

Detection of Human HOMER1 by Western Blot.

Western blot shows lysates of human brain (cerebellum) tissue and IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-HOMER1 Monoclonal Antibody (Catalog # MAB6889) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HOMER1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
Detection of Human and Mouse HOMER1 antibody by Simple WesternTM.

Detection of Human and Mouse HOMER1 by Simple WesternTM.

Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for HOMER1 at approximately 51 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat HOMER1 Monoclonal Antibody (Catalog # MAB6889). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for HOMER1 Antibody (666241)

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: human brain (cerebellum) tissue

Western Blot

2 µg/mL
Sample: Human brain (cerebellum) tissue and IMR‑32 human neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HOMER1

HOMER1 is an approximately 48 kDa cytoplasmic protein that regulates the cell surface localization of neuronal mGluR1 and mGluR5 metabotropic glutamate receptors. It also functions in striated muscle mechanotransduction and excitation-contraction coupling by regulating the localization of transient receptor potential (TRP) channels and the coupling of ryanodine receptors to Cav1.2 calcium channels. Alternately spliced isoforms of human HOMER1 lack either the C-terminal half of the molecule (aa 178-354) or 130 aa following the WH1 domain (aa 99-228). Particular isoforms show distinct effects on hippocampal neuronal plasticity and the maintenance of extracellular glutamate levels in the prefrontal cortex. Within aa 73-82, human HOMER1 shares 100% aa sequence identity with mouse and rat HOMER1.

Long Name

Homer Protein Homolog 1

Alternate Names

HOMER1A, HOMER1B, HOMER1C, SYN47, Ves-1

Entrez Gene IDs

9456 (Human); 26556 (Mouse)

Gene Symbol

HOMER1

UniProt

Q86YM7

Additional HOMER1 Products

Product Documents for HOMER1 Antibody (666241)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for HOMER1 Antibody (666241)

For research use only

Related Research Areas

Neuronal Markers

Citations for HOMER1 Antibody (666241)

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