Human/Mouse/Rat Importin beta /KPNB1 Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB8209T Datasheet / COA / SDS

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FAB8209T-100UG

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Human/Mouse/Rat Importin beta /KPNB1 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human Importin beta /KPNB1 in ELISA and Western Blot. It detects mouse and rat Importin beta /KPNB1 in Western Blots.

Source

Monoclonal Mouse IgG2b Clone # 845208

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Importin Î²/KPNB1
Met1-Gly155
Accession # Q14974

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Importin beta/KPNB1

KPNB1 (Karyopherin subunit beta 1), also called Importin beta 1, NTF97 (nuclear transport factor, 97 kDa) or PTAC97 (pore targeting complex, 97 kDa), is a member of the Importin b family of proteins. It is ubiquitously expressed and plays many roles in the nucleus. Primarily, KPNB1 transports various complexes from cytoplasm into nucleus, either alone or with a KPNA adaptor. The 876 amino acid (aa) human KPNB1 contains an N-terminal IBB/Importin b domain (aa 21-101), and eight HEAT (solenoid) domains (aa 124-726). A 731 aa splice variant begins with Met146, while an 845 aa variant shows a 2 aa substitution for aa 702-734. Binding sites for adaptors and for its regulator, Ran-GTP, overlap within the HEAT domains. Human KPNB1 aa 1-155 shares 99% and 97% aa sequence identity with mouse and rat KPNB1, respectively.

Long Name

Karyopherin beta 1

Entrez Gene IDs

3837 (Human); 16211 (Mouse); 24917 (Rat)

Alternate Names

IMB1; Impnb; Importin 1; importin 90; Importin beta; importin beta-1 subunit; importin subunit beta-1; importin-90; IPO1; IPOB; karyopherin (importin) beta 1; Karyopherin subunit beta-1; KPNB1; MGC2155; MGC2156; MGC2157; NTF97; NTF97Pore targeting complex 97 kDa subunit; Nuclear factor p97; PTAC97