Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 413104
View all Jak1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Jak1
Pro32-Phe286
Accession # P23458

Specificity

Detects human, mouse, and rat Jak1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat Jak1 Antibody

Detection of Human and Mouse Jak1 antibody by Western Blot.

Detection of Human and Mouse Jak1 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, A20 mouse B cell lymphoma cell line, and L1.2 mouse pro-B cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse/Rat Jak1 Monoclonal Antibody (Catalog # MAB4260) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Jak1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Detection of Human/Mouse/Rat Jak1 by Simple WesternTM.

Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Jak1 at approximately 136 kDa (as indicated) using 10 µg/mL of Rat Anti-Human/Mouse/Rat Jak1 Monoclonal Antibody (Catalog # MAB4260). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat Jak1 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Lysates of Daudi human Burkitt's lymphoma cell line.

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, A20 mouse B cell lymphoma cell line, and L1.2 mouse pro-B cell line

Reviewed Applications

Read 4 reviews rated 5 using MAB4260 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Jak1

Janus Kinase 1 (Jak1) belongs to a family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Activation of Jak1 occurs via phosphorylation at two adjacent tyrosine residues, Y1022 and Y1023, within the kinase domain. Jaks activate members of the STAT family of transcription factors by phosphorylating critical tyrosine regulatory sites. Jak1 is required for the activation of STAT1 and STAT2 in response to interferon alpha.

Long Name

Janus Kinase 1

Alternate Names

EC 2.7.10, JAK-1, JAK1Atyrosine-protein kinase JAK1, JAK1B, Janus kinase 1EC 2.7.10.2, JTK3

Entrez Gene IDs

3716 (Human)

Gene Symbol

JAK1

UniProt

P23458

Additional Jak1 Products

Product Documents for Human/Mouse/Rat Jak1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat Jak1 Antibody

This product is sold under license from Millipore Corporation under the following US or foreign patents: 5,821,069; 5,658,791; EP0560890. This product shall not be used to commercially screen drug molecules being developed as JAK1 or JAK2 inhibitors. Any such activity will be outside the scope of the research use only label license.

For research use only

Citations for Human/Mouse/Rat Jak1 Antibody

Customer Reviews for Human/Mouse/Rat Jak1 Antibody (4)

5 out of 5
4 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

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  • Human/Mouse/Rat Jak1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Skin tissue
    Species: Mouse
    Verified Customer | Posted 02/15/2022
    Human/Mouse/Rat Jak1 Antibody MAB4260
  • Human/Mouse/Rat Jak1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Lung adenocarcinoma
    Species: Human
    Verified Customer | Posted 11/05/2021
    Human/Mouse/Rat Jak1 Antibody MAB4260
  • Human/Mouse/Rat Jak1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Breast cancer tissue
    Species: Human
    Verified Customer | Posted 04/04/2020
    Human/Mouse/Rat Jak1 Antibody MAB4260
  • Human/Mouse/Rat Jak1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Cancer Tissue
    Species: Human
    Verified Customer | Posted 10/17/2018
    Human/Mouse/Rat Jak1 Antibody MAB4260

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FAQs for Human/Mouse/Rat Jak1 Antibody

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    • Q: Will the Human/Mouse/Rat Jak1 Antibody, Catalog # MAB4260, detect both phosphorylated and unphosphorylated JAK1?
      A: The Human/Mouse/Rat Jak1 Antibody, Catalog # MAB4260,  was raised against an E. coli-derived recombinant human Jak1 (Pro32-Phe286) protein, Accession # P23458. The epitope detected by the antibody is in this region. Theoretically, the antibody would react with both phosphorylated and unphosphorylated JAK1.  However, we have not attempted to confirm this experimentally.
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