NLRP10, also known as Pynod, NALP10, and NOD8, is a 75 kDa cytoplasmic protein that associates with the ASC component of inflammasomes. NLRP10 inhibits ASC‑dependent apoptosis, NFkB activation, and Caspase-1-dependent processing of IL-1 beta. Human NLRP10 contains one Pyrin domain (aa 1-96) and one NACHT domain (aa 167-484) but no leucine-rich repeats. Within aa 386-655, human NLRP10 shares approximately 50% aa sequence identity with mouse and rat NLRP10.
NLRP10/Pynod/NALP10 Antibody (578502)
R&D Systems | Catalog # MAB6606
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 578502
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Product Specifications
Immunogen
E. coli-derived recombinant human NLRP10/Pynod/NALP10
Met386-Ile655
Accession # Q86W26
Met386-Ile655
Accession # Q86W26
Specificity
Detects human NLRP10/Pynod/NALP10 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) NALP1 (aa 1‑323), rhNALP1 (aa 1331‑1429), rhNALP2 (aa 1‑208), rhNALP3 (aa 3‑223), rhNALP3 (aa 540‑689), rhNALP5 (aa 469‑635), rhNOD1 (aa 830‑953), or rhNOD2 (aa 230‑450) is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for NLRP10/Pynod/NALP10 Antibody (578502)
Detection of Human, Mouse, and Rat NLRP10/Pynod/NALP10 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HT-2 mouse T cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Human/Mouse/Rat NLRP10/Pynod/NALP10 Monoclonal Antibody (Catalog # MAB6606) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NLRP10/Pynod/ NALP10 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for NLRP10/Pynod/NALP10 Antibody (578502)
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, HT‑2 mouse T cell line, and L6 rat myoblast cell line
Sample: Jurkat human acute T cell leukemia cell line, HT‑2 mouse T cell line, and L6 rat myoblast cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: NLRP10/Pynod/NALP10
Long Name
NLR Family, Pyrin Domain Containing 10
Alternate Names
NALP10, NOD8, PAN5, Pynod
Gene Symbol
NLRP10
UniProt
Additional NLRP10/Pynod/NALP10 Products
Product Documents for NLRP10/Pynod/NALP10 Antibody (578502)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for NLRP10/Pynod/NALP10 Antibody (578502)
For research use only
Related Research Areas
Citations for NLRP10/Pynod/NALP10 Antibody (578502)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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