PIM2 Antibody (447023)

R&D Systems | Catalog # MAB4355

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 447023
View all PIM2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PIM2
Met1-Pro311
Accession # Q9P1W9

Specificity

Detects human, mouse, and rat PIM2 in Western blots. In Western blots, no cross-reactivity with recombinant human PIM1 or PIM3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for PIM2 Antibody (447023)

Detection of Human PIM2 antibody by Western Blot.

Detection of Human PIM2 by Western Blot.

Western blot shows Recombinant Human Active PIM1 (Catalog # 4588-KS), recombinant human PIM2, and recombinant human PIM3 (5 ng/lane). PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/ Mouse/Rat PIM2 Monoclonal Antibody (Catalog # MAB4355) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for PIM2 was detected at approximately 38-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Detection of Human/Mouse/Rat PIM2 antibody by Western Blot.

Detection of Human/Mouse/Rat PIM2 by Western Blot.

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, Raji human Burkitt's lymphoma cell line, SW480 human colorectal adenocarcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat PIM2 Monoclonal Antibody (Catalog # MAB4355) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for PIM2 was detected at approximately 38 kDa and 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for PIM2 Antibody (447023)

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HT-29 human colon adenocarcinoma cell line, Raji human Burkitt's lymphoma cell line, SW480 human colorectal adenocarcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and rat brain tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PIM2

The three members of the Pim family of proto-oncogenes encode the serine/threonine kinases PIM1, PIM2, and PIM3. Multiple isoforms of PIM2 are expressed in many tissues, with high levels in spleen, thymus, testis, small intestine, and colon. PIM2 is implicated as a mediator of IL-3 survival signaling in hematopoietic cells, and dysregulated PIM2 expression is observed in prostate cancer, lymphoma, leukemia, and multiple myeloma.

Long Name

Proviral Integration Site 2

Alternate Names

EC 2.7.11.1, pim-2 oncogene, Pim-2h, proto-oncogene Pim-2 (serine threonine kinase), serine/threonine protein kinase pim-2, serine/threonine-protein kinase pim-2

Entrez Gene IDs

11040 (Human); 18715 (Mouse); 317366 (Rat)

Gene Symbol

PIM2

UniProt

Q9P1W9

Additional PIM2 Products

Product Documents for PIM2 Antibody (447023)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for PIM2 Antibody (447023)

For research use only

Related Research Areas

Epigenetics

Citations for PIM2 Antibody (447023)

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