PINCH1 Antibody (591604)

R&D Systems | Catalog # MAB6257

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Fish - Danio rerio (Zebrafish)

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 591604
View all PINCH1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PINCH1
Ala2-Gly108
Accession # P48059

Specificity

Detects human, mouse, and rat PINCH1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for PINCH1 Antibody (591604)

Detection of Human and Rat PINCH1 antibody by Western Blot.

Detection of Human and Rat PINCH1 by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line, WI-38 human lung fibroblast cell line, HepG2 human hepatocellular carcinoma cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PINCH1 Monoclonal Antibody (Catalog # MAB6257) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for PINCH1 at approximately 37-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for PINCH1 Antibody (591604)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: A549 human lung carcinoma cell line, WI‑38 human lung fibroblast cell line, HepG2 human hepatocellular carcinoma cell line, and NRK rat normal kidney cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PINCH1

Particularly interesting new Cys-His protein 1 (PINCH1), also known as LIMS1 (LIM and senescent cell antigen-like-containing domain protein 1), is a 37‑40 kDa, widely expressed LIM-only protein. Human PINCH1 is 325 amino acids (aa) in length and contains a tandem array of five LIM domains. Within the region used as an immunogen, human PINCH1 shares 100% and 99% aa sequence identity with mouse and rat PINCH1, respectively. PINCH1 is an effector of integrin and growth factor signaling, coupling surface receptors to downstream signaling molecules involved in the regulation of cell survival, cell proliferation and cell differentiation.

Long Name

Particularly Interesting New Cys-His Protein 1

Alternate Names

LIMS1

Entrez Gene IDs

3987 (Human)

Gene Symbol

LIMS1

UniProt

P48059 (Human)

Additional PINCH1 Products

Product Documents for PINCH1 Antibody (591604)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for PINCH1 Antibody (591604)

For research use only

Related Research Areas

Integrins and Associated Molecules

Citations for PINCH1 Antibody (591604)

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