Human/Mouse/Rat SMURF2 Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB6916N Datasheet / COA / SDS

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FAB6916N-100UG

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Human/Mouse/Rat SMURF2 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human SMURF2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human SMURF1 is observed.

Source

Monoclonal Mouse IgG2a Clone # 690638

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human SMURF2
Met490-Glu748
Accession # Q9HAU4

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SMURF2

SMURF2 (SMAD ubiquitin regulatory factor-2) is a ~86 kDa, 748 amino acid (aa) cytoplasmic E3 ubiquitin ligase that negatively regulates TGF-beta signaling by promoting proteasomal degradation of some SMAD signaling molecules. It also influences cell cycle progression, peaking during mitosis and binding MAD2L1 and NEDD9/CASL. SMURF2 contains C2, WW1-3, and HECT domains. A potential 735 aa form lacks aa 19-31 (within the C2 domain), while a 507 aa form shows an alternate start site at aa 242 (between WW1 and WW2). Within the HECT domain region used as an immunogen, human SMURF2 shares 99% and 98% aa identity with mouse and rat SMURF2, respectively.

Long Name

SMAD Ubiquitination Regulatory Factor 2

Entrez Gene IDs

64750 (Human); 66313 (Mouse); 303614 (Rat)

Alternate Names

DKFZp686F0270; E3 ubiquitin ligase SMURF2; E3 ubiquitin-protein ligase SMURF2; EC 6.3.2; EC 6.3.2.-; hSMURF2; MGC138150; SMAD specific E3 ubiquitin protein ligase 2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2; SMURF2