SMURF2 Antibody (690638)

R&D Systems | Catalog # MAB6916

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 690638
View all SMURF2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SMURF2
Met490-Glu748
Accession # Q9HAU4

Specificity

Detects human SMURF2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human SMURF1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for SMURF2 Antibody (690638)

Detection of Human, Mouse, and Rat SMURF2 antibody by Western Blot.

Detection of Human, Mouse, and Rat SMURF2 by Western Blot.

Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SMURF2 Monoclonal Antibody (Catalog # MAB6916) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SMURF2 at approximately 93 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for SMURF2 Antibody (690638)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: MDA‑MB‑468 human breast cancer cell line, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SMURF2

SMURF2 (SMAD ubiquitin regulatory factor-2) is a ~86 kDa, 748 amino acid (aa) cytoplasmic E3 ubiquitin ligase that negatively regulates TGF-beta signaling by promoting proteasomal degradation of some SMAD signaling molecules. It also influences cell cycle progression, peaking during mitosis and binding MAD2L1 and NEDD9/CASL. SMURF2 contains C2, WW1-3, and HECT domains. A potential 735 aa form lacks aa 19-31 (within the C2 domain), while a 507 aa form shows an alternate start site at aa 242 (between WW1 and WW2). Within the HECT domain region used as an immunogen, human SMURF2 shares 99% and 98% aa identity with mouse and rat SMURF2, respectively.

Long Name

SMAD Ubiquitination Regulatory Factor 2

Alternate Names

DKFZp686F0270, E3 ubiquitin ligase SMURF2, E3 ubiquitin-protein ligase SMURF2, EC 6.3.2, EC 6.3.2.-, hSMURF2, MGC138150, SMAD specific E3 ubiquitin protein ligase 2, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2

Entrez Gene IDs

64750 (Human); 66313 (Mouse); 303614 (Rat)

Gene Symbol

SMURF2

UniProt

Q9HAU4

Additional SMURF2 Products

Product Documents for SMURF2 Antibody (690638)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for SMURF2 Antibody (690638)

For research use only

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways
TGF-beta Signaling Pathway Thumbnail