Human/Mouse/Rat Syntaxin 7 Alexa Fluor® 700-conjugated Antibody

Catalog #: AF5478N Datasheet / COA / SDS

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AF5478N-100UG

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Human/Mouse/Rat Syntaxin 7 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat Syntaxin 7 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Syntaxin 7
Asn21-Glu187
Accession # O15400

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Syntaxin 7

Syntaxin 7 (STX7) is a widely expressed protein embedded in endosomal and lysosomal membranes, and serves as a component of the SNARE complex. STX7 is involved in endocytic trafficking from early endosomes to late endosomes and lysosomes. This is in contrast to STX8, which is involved in clathrin-independent vesicular transport. Human STX7 is a type IV single-pass transmembrane protein (very short exoplasmic C-terminus) that is 261 amino acids (aa) in length. It contains a coiled-coil region (aa 47‑69), a t-SNARE domain (aa 165‑227) that is likely involved in protein-protein interactions, and a short, two amino acid, C-terminal luminal sequence. Over aa 21‑187, human STX7 shares 97% aa identity with mouse STX7.

Entrez Gene IDs

8417 (Human); 53331 (Mouse); 60466 (Rat)

Alternate Names

STX7; Syntaxin 7; syntaxin-7