Human/Mouse/Rat TOR Antibody

Name: Human/Mouse/Rat TOR Antibody
Brand: R&D Systems
SKU: AF15371
Price: 489 USD
Availability: InStock

R&D Systems | Catalog # AF15371

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Citations (7)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all TOR Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TOR
Phe1720-Ala2020
Accession # P42345

Specificity

Detects human, mouse, and rat TOR.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat TOR Antibody

Detection of Human/Mouse/Rat TOR by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat TOR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF15371) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TOR at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat TOR Antibody

Application

Recommended Usage

Immunoprecipitation

2 µg/500 µg cell lysate
Sample: MCF‑7 human breast cancer cell line, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: NRK rat normal kidney cell, MCF-7 human breast cancer cell line, and Balb/3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TOR

The Target of Rapamycin (TOR) is a member of the PI 3-kinase-related kinase (PIKK) family. TOR is the protein target of rapamycin, an anti-rejection drug used in transplantation and promising anti-cancer agent. TOR plays a crucial role in the control of cell growth and proliferation as a downstream target of the PI 3-kinase/Akt signal transduction pathway.