Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 513710
View all STAT4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human STAT4
Leu83-Lys162
Accession # Q14765

Specificity

Detects endogenous human STAT4 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse STAT4 Antibody

Detection of Human STAT4 antibody by Western Blot.

Detection of Human STAT4 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and NC-37 human Burkitt's lymphoma B lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse STAT4 Monoclonal Antibody (Catalog # MAB5287) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STAT4 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse STAT4 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and NC-37 human Burkitt's lymphoma B lymphoblast cell line

Reviewed Applications

Read 3 reviews rated 4 using MAB5287 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: STAT4

Signal transduction and activator of transcription 4 (STAT4) is a 86 kDa member of the Jak/STAT signal transduction pathway. Human STAT4 is 748 amino acids (aa) in length. STAT4 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, homo- or heterodimerizes with other STAT proteins, which then translocate to the nucleus and activate transcription of specific genes. STAT4 contains a SH2 domain (aa 569‑664) and a Jak1 phophorylation site at Y693. STAT4 is involved in mediating IL‑12 response in lymphocytes and regulating T helper cell differentiation, and STAT4 variants are found in patients with systemic lupus erythematosus.

Long Name

Signal Transducer and Activator of Transcription 4

Alternate Names

signal transducer and activator of transcription 4, SLEB11

Entrez Gene IDs

6775 (Human); 20849 (Mouse)

Gene Symbol

STAT4

UniProt

Q14765

Additional STAT4 Products

Product Documents for Human/Mouse STAT4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse STAT4 Antibody

For research use only

Citations for Human/Mouse STAT4 Antibody

Customer Reviews for Human/Mouse STAT4 Antibody (3)

4 out of 5
3 Customer Ratings
5 Stars
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4 Stars
100%
3 Stars
0%
2 Stars
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1 Stars
0%

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  • Human/Mouse STAT4 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    Human/Mouse STAT4 Antibody MAB5287
  • Human/Mouse STAT4 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Plasma
    Species: Human
    Verified Customer | Posted 11/10/2018
  • Human/Mouse STAT4 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/09/2018

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