STAT4 Antibody (513710)

R&D Systems | Catalog # MAB5287

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 513710
View all STAT4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human STAT4
Leu83-Lys162
Accession # Q14765

Specificity

Detects endogenous human STAT4 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for STAT4 Antibody (513710)

Detection of Human STAT4 antibody by Western Blot.

Detection of Human STAT4 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and NC-37 human Burkitt's lymphoma B lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse STAT4 Monoclonal Antibody (Catalog # MAB5287) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STAT4 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for STAT4 Antibody (513710)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and NC-37 human Burkitt's lymphoma B lymphoblast cell line

Reviewed Applications

Read 3 reviews rated 4 using MAB5287 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: STAT4

Signal transduction and activator of transcription 4 (STAT4) is a 86 kDa member of the Jak/STAT signal transduction pathway. Human STAT4 is 748 amino acids (aa) in length. STAT4 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, homo- or heterodimerizes with other STAT proteins, which then translocate to the nucleus and activate transcription of specific genes. STAT4 contains a SH2 domain (aa 569‑664) and a Jak1 phophorylation site at Y693. STAT4 is involved in mediating IL‑12 response in lymphocytes and regulating T helper cell differentiation, and STAT4 variants are found in patients with systemic lupus erythematosus.

Long Name

Signal Transducer and Activator of Transcription 4

Alternate Names

signal transducer and activator of transcription 4, SLEB11

Entrez Gene IDs

6775 (Human); 20849 (Mouse)

Gene Symbol

STAT4

UniProt

Q14765

Additional STAT4 Products

Product Documents for STAT4 Antibody (513710)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for STAT4 Antibody (513710)

For research use only

Citations for STAT4 Antibody (513710)

Customer Reviews for STAT4 Antibody (513710) (3)

4 out of 5
3 Customer Ratings
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used STAT4 Antibody (513710)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 3 的 3 reviews Showing All
Filter By:
Clear Filters X
  • Human/Mouse STAT4 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    STAT4 Antibody (513710) MAB5287
  • Human/Mouse STAT4 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Plasma
    Species: Human
    Verified Customer | Posted 11/10/2018
  • Human/Mouse STAT4 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/09/2018

There are no reviews that match your criteria.

Showing  1 - 3 的 3 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies