SWAP70 Antibody (683317)

R&D Systems | Catalog # MAB6735

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 683317
View all SWAP70 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SWAP70
Arg386-Glu585
Accession # Q9UH65

Specificity

Detects human SWAP70 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for SWAP70 Antibody (683317)

Detection of Human and Mouse SWAP70 antibody by Western Blot.

Detection of Human and Mouse SWAP70 by Western Blot.

Western blot shows lysates of BJAB human Burkitt's lymphoma cell line and L1.2 mouse pro-B cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse SWAP70 Monoclonal Antibody (Catalog # MAB6735) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SWAP70 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for SWAP70 Antibody (683317)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: BJAB human Burkitt's lymphoma cell line and L1.2 mouse pro-B cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SWAP70

SWAP70 (Switch-associated protein 70) is a 67-70 kDa intracellular protein that bears limited resemblance to members of the Dbl-family of GEFs for small Rho GTPases. It is expressed in mast cells, dendritic cells and B cells. In mast cells, it promotes the release of anaphylactic mediators; in dendritic cells, it regulates the appearance of MHC II; and in B cells, it forms a nuclear complex that initiates immunoglobulin class switching. Human SWAP70 is 585 amino acids (aa) in length. It contains one plextrin homology (PH) domain (aa 213-304) plus a coiled-coil region (aa 316-539). There are three potential splice variants. One contains an alternative start site at Met70, another shows a deletion of aa 81-138, and a third possesses a 38 aa substitution for aa 453-585. Over aa 386-585, human SWAP70 shares 96% aa identity with mouse SWAP70.

Long Name

Switch-associated Protein 70 kDa

Alternate Names

KIAA0640SWAP-70FLJ39540, SWAP switching B-cell complex 70kDa subunit, switch-associated protein 70

Entrez Gene IDs

23075 (Human); 20947 (Mouse); 293410 (Rat)

Gene Symbol

SWAP70

UniProt

Q9UH65

Additional SWAP70 Products

Product Documents for SWAP70 Antibody (683317)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for SWAP70 Antibody (683317)

For research use only

Related Research Areas

B Cell Activation

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