Human Musashi-1 Antibody (282613)

R&D Systems | Catalog # MAB2628

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 282613
View all Musashi-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Musashi-1
aa 1-362

Specificity

Detects human Musashi-1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Musashi-1 Antibody (282613)

Detection of Human Musashi-1 antibody by Western Blot.

Detection of Human Musashi-1 by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human Musashi-1 Monoclonal Antibody (Catalog # MAB2628) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Musashi-1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Musashi-1 Antibody (282613)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Musashi-1

Musashi-1 is an RNA-binding protein that is expressed in the developing central nervous system. Expression is highest in proliferating multipotent neural precursor cells and decreases during neuronal differentiation (1, 2).

References

  1. Kaneko, Y. et al. (2000) Dev. Neurosci. 22:139.
  2. Kanemura, Y. et al. (2002) Meth. Mol. Biol. 198:273.

Alternate Names

MSI1, Musashi1

Entrez Gene IDs

4440 (Human)

Gene Symbol

MSI1

Additional Musashi-1 Products

Product Documents for Human Musashi-1 Antibody (282613)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Musashi-1 Antibody (282613)

For research use only

Citations for Human Musashi-1 Antibody (282613)

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