Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Confirmation of Specificity using RNAscope ISH

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 985917
View all NF-L Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NF-L
Met1-Asp338
Accession # P07196

Specificity

Detects human NF-L in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human NF‑L Antibody

NF‑L in U‑251 MG Human Cell Line.

NF‑L was detected in immersion fixed U‑251 MG human glioblastoma cell line (positive staining) and K562 human chronic myelogenous leukemia cell line (negative staining) using Mouse Anti-Human NF‑L Monoclonal Antibody (Catalog # MAB22162) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

NF-L in U-251 MG human glioblastoma cell line using RNAscope®ISH

NF-L mRNA was detected in formalin-fixed U-251 MG human glioblastoma cell line probed with ACD RNAScope®Probe-Hs-NEFL (Catalog # 468671) and stained using ACD RNAscope®2.5 HD Detection Reagents-RED (Catalog # 32260). This is an In Situ Hybridization control for NF-L expression.

Applications for Human NF‑L Antibody

Application
Recommended Usage

Confirmation of Specificity using RNAscope ISH

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed U-251 MG human glioblastoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB22162 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NF-L

NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that is expressed in neurons. It associates with the 125 kDa NF-M and the 200 kDa NF-H to form neurofilaments.

Long Name

Neurofilament Protein, Light Chain

Alternate Names

CMT2E, NEFL, NF68, NFL

Entrez Gene IDs

4747 (Human)

Gene Symbol

NEFL

UniProt

P07196

Additional NF-L Products

Product Documents for Human NF‑L Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NF‑L Antibody

For research use only

Customer Reviews for Human NF‑L Antibody (1)

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  • Human NF-L Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 12/03/2021
    Human NF‑L Antibody MAB22162

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