Human NFIL3/E4BP4 Antibody

Catalog #: MAB11657 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11657-100
MAB11657-SP

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Detection of Human NFIL3/E4BP4 by Western Blot.

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Human NFIL3/E4BP4 Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human NFIL3 in Direct ELISA.

Source

Monoclonal Mouse IgG_2A Clone # 1095812

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human NFIL-3
Lys140-Gly462
Accession # Q16649

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

HepG2 human hepatocellular carcinoma cell line

Simple Western

20 µg/mL

HepG2 human hepatocellular carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human NFIL3/E4BP4 by Western Blot. Western Blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human NFIL3/E4BP4 Monoclonal Antibody (Catalog # MAB11657) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NFIL3/E4BP4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western

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Detection of Human NFIL3/E4BP4 by Simple Western^TM. Simple Western shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for NFIL3/E4BP4 at approximately 63 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human NFIL3/E4BP4 Monoclonal Antibody (Catalog # MAB11657). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NFIL3/E4BP4

NFIL3 (Nuclear Factor, Interleukin 3 Regulated), also called E4BP4 (adenovirus E4 promoter binding protein 4), is an approximately 58 kDa transcription factor of the bZIP (basic leucine zipper) family. It is a transcriptional regulator expressed in T and pro-B lymphocytes and cardiomyocytes, binds IL-3 and promoters as a homodimer, and enhances cell survival. NFIL3 has also been shown to be involved in the development of Innate Lymphoid Cells (ILCs; 1,2). The 462 amino acid (aa) human NFIL3 contains a basic DNA binding domain (aa 73-95), a leucine zipper (aa 96-124), and a transcriptional repression domain (aa 299-363) that is potentially regulated by phosphorylation at Ser301 and Ser353. Within the region used as an immunogen, human NFIL3 shares 83% aa sequence identity with mouse and rat NFIL3.

References

Seillet, C. et al. (2014) J Exp Med. 211:1733.
Geiger, T.L. et al. (2014) J Exp Med. 211:1723.

Long Name

Nuclear Factor, Interleukin 3 Regulated

Entrez Gene IDs

4783 (Human); 18030 (Mouse); 114519 (Rat)

Alternate Names

E4BP4; E4BP4IL3BP1; IL3BP1; Interleukin-3 promoter transcriptional activator; Interleukin-3-binding protein 1; NFIL3; NF-IL3A; NFIL3AE4 promoter-binding protein 4; nuclear factor interleukin 3 regulated protein; nuclear factor interleukin-3-regulated protein; nuclear factor, interleukin 3 regulated; Transcriptional activator NF-IL3A