Human NFIL3/E4BP4 Antibody (1095812)

R&D Systems | Catalog # MAB11657

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1095812
View all NFIL3/E4BP4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NFIL-3
Lys140-Gly462
Accession # Q16649

Specificity

Detects recombinant human NFIL3 in Direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human NFIL3/E4BP4 Antibody (1095812)

Detection of Human NFIL3/E4BP4 by Western Blot.

Western Blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human NFIL3/E4BP4 Monoclonal Antibody (Catalog # MAB11657) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NFIL3/E4BP4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human NFIL3/E4BP4 by Simple WesternTM.

Simple Western shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for NFIL3/E4BP4 at approximately 63 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human NFIL3/E4BP4 Monoclonal Antibody (Catalog # MAB11657). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human NFIL3/E4BP4 Antibody (1095812)

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Western Blot

2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NFIL3/E4BP4

NFIL3 (Nuclear Factor, Interleukin 3 Regulated), also called E4BP4 (adenovirus E4 promoter binding protein 4), is an approximately 58 kDa transcription factor of the bZIP (basic leucine zipper) family. It is a transcriptional regulator expressed in T and pro-B lymphocytes and cardiomyocytes, binds IL-3 and promoters as a homodimer, and enhances cell survival. NFIL3 has also been shown to be involved in the development of Innate Lymphoid Cells (ILCs; 1,2). The 462 amino acid (aa) human NFIL3 contains a basic DNA binding domain (aa 73-95), a leucine zipper (aa 96-124), and a transcriptional repression domain (aa 299-363) that is potentially regulated by phosphorylation at Ser301 and Ser353. Within the region used as an immunogen, human NFIL3 shares 83% aa sequence identity with mouse and rat NFIL3.

References

  1. Seillet, C. et al. (2014) J Exp Med. 211:1733.
  2. Geiger, T.L. et al. (2014) J Exp Med. 211:1723.

Long Name

Nuclear Factor, Interleukin 3 Regulated

Alternate Names

E4BP4, IL3BP1, NF-IL3A

Entrez Gene IDs

4783 (Human); 18030 (Mouse); 114519 (Rat)

Gene Symbol

NFIL3

UniProt

Q16649

Additional NFIL3/E4BP4 Products

Product Documents for Human NFIL3/E4BP4 Antibody (1095812)

Certificate of Analysis

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Product Specific Notices for Human NFIL3/E4BP4 Antibody (1095812)

For research use only

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