Human PARD3/Par3 Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB8030N Datasheet / COA / SDS

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FAB8030N-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PARD3/Par3 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human PARD3/Par3 in ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human PARD3b/Par3b is observed.

Source

Monoclonal Mouse IgG1 Clone # 833517

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human PARD3/Par3
Tyr451-Glu555
Accession # Q8TEW0

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PARD3/Par3

PARD3 (partitioning defective 3 homolog), also called Par3, is a widely expressed adapter protein involved in asymmetrical cell division, cell polarization, and tight junction formation. It forms a cell polarity complex with Par6 and aPKC (atypical protein kinase C), linking to PTEN (phosphatase and tensin homolog) in polarized epithelia and VE-Cadherin in polarized endothelia. The region used as an immunogen, human PARD3 amino acids (aa) 451-555 (of 1356 aa in full-length PARD3) shares 97% and 96% aa sequence identity with mouse and rat PARD3, respectively. It includes the second of three PDZ domains, a region which is involved in heterophilic interactions and is present within all 10 reported PARD3 isoforms that vary in length from 988 aa to 1356 aa.

Long Name

Par-3 Partitioning Defective 3 Homolog

Entrez Gene IDs

56288 (Human); 93742 (Mouse); 81918 (Rat)

Alternate Names

ASIP; Baz; Bazooka; FLJ21015; par-3 partitioning defective 3 homolog (C. elegans); PAR3; PAR3A; PAR3C.elegans) homolog; PARD3; SE2-5L16; SE2-5LT1; SE2-5T2