Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is an enzyme encoded by the NOS3 gene. Endothelial NOS generates nitric oxide in blood vessels and is involved with regulating vascular tonehttp://en.wikipedia.org/wiki/Vascular_resistance" title="Vascular resistance"> by inhibiting smooth muscle contraction and platelethttp://en.wikipedia.org/wiki/Platelet" title="Platelet"> aggregation. A constitutive calciumhttp://en.wikipedia.org/wiki/Calcium_in_biology" title="Calcium in biology"> dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of the Golgi apparatushttp://en.wikipedia.org/wiki/Golgi_apparatus" title="Golgi apparatus"> within cells.
Human phospho-eNOS (S1177) Antibody (1014F)
R&D Systems | Catalog # MAB9028
Recombinant Monoclonal Antibody.
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Simple Western
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1014F
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Product Specifications
Immunogen
Phosphopeptide containing the human eNOS S1177 site
Accession # P29474
Accession # P29474
Specificity
Detects human eNOS when phosphorylated at S1177 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human phospho-eNOS (S1177) Antibody (1014F)
Detection of Human eNOS by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 100 µM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-eNOS (S1177) Monoclonal Antibody (Catalog # MAB9028) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for eNOS at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human eNOS by Simple WesternTM.
Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with Pervanadate (PV), loaded at 0.2 mg/mL. A specific band was detected for eNOS at approximately 141 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Phospho-eNOS (S1177) Monoclonal Antibody (Catalog # MAB9028). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.Applications for Human phospho-eNOS (S1177) Antibody (1014F)
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: HUVEC human umbilical vein endothelial cells treated with Pervanadate
Sample: HUVEC human umbilical vein endothelial cells treated with Pervanadate
Western Blot
0.5 µg/mL
Sample: HUVEC human umbilical vein endothelial cells treated with Pervanadate
Sample: HUVEC human umbilical vein endothelial cells treated with Pervanadate
Reviewed Applications
Read 2 reviews rated 5 using MAB9028 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: eNOS
Long Name
Endothelial Nitric Oxide Synthase
Alternate Names
NOS3
Gene Symbol
NOS3
UniProt
Additional eNOS Products
Product Documents for Human phospho-eNOS (S1177) Antibody (1014F)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human phospho-eNOS (S1177) Antibody (1014F)
For research use only
Related Research Areas
Citations for Human phospho-eNOS (S1177) Antibody (1014F)
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Customer Reviews for Human phospho-eNOS (S1177) Antibody (1014F) (2)
5 out of 5
2 Customer Ratings
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Application: Western BlotSample Tested: Human aortic endothelial cellsSpecies: HumanVerified Customer | Posted 01/19/2019
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Application: Western BlotSample Tested: Heart tissueSpecies: Guinea PigVerified Customer | Posted 09/08/2017Used 1/500 dilution for WB and obtained a very clean and clear individual band at the expected MW. Regular Bis Tris Criterion Gel electrophoresis from BioRad was run with MES buffer. Immobilion transfer membrane purchased from Millipore.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
