Human Phospho-FAK (Y925) Alexa Fluor® 647-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: FAK

Focal adhesion kinase 1 (FAK) is a ubiquitously expressed nonreceptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a "Focal Adhesion Targeting" (FAT) sequence, a 125 amino acid sequence at the C-terminus. FAK plays an important role in migration, cell spreading, differentiation, cytoskeleton protein phosphorylation, apoptosis and acceleration of the G1 to S phase transition of the cell cycle. Increased expression and/or activity of FAK in various tumors has been correlated with enhanced migration and invasiveness of human tumor cells in addition to promoting increased cell proliferation. Src-dependent phosphorylation of FAK at Tyr925 regulates the effect of FAK on cell migration and tumor angiogenesis. Human FAK contains one FERM domain (aa 35-355) and one kinase domain (aa 415-684). Alternate splicing generates additional isoforms with N-terminal and C-terminal truncations. Within aa 919-930 human, mouse, and rat FAK share 100% aa sequence identity.

Product Datasheets

Related Research Areas

• Adhesion Molecules on Endothelial Cells
• Adhesion Molecules on VSMC
• Chemokine Receptor Intracellular Signaling
• Focal Adhesions
• GDNF Family
• Intracellular Kinases in the Akt Pathway
• Intracellular Signaling Molecules in Angiogenesis
• Kinases and Phosphatases in Endothelial Cells
• Kinases and Phosphatases in VSMC
• Mesenchymal Cells and EMT
• Platelet Signaling
• Repulsive Guidance Molecules and Receptors
• VEGF Family