Human phospho-p27/Kip1 (T198) Antibody

Detection of Human Phospho-p27/Kip1 (T198) by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, non-transfected, transfected with plasmid expressing YFP-p27/Kip1 wild type (WT), or transfected with YFP-p27/Kip1 T198A mutant (T198A). PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-p27/Kip1 (T198) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3994) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p27/Kip1 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Phospho-p27/Kip1 (T198) by Western Blot

STAT3/cJun/p27 co-repress PTPN12 in complex with YY1, SIN3A, and HDAC1.a Volcano plot of genes differentially expressed in 231DD vs. 231. bPTPN12 mRNA expression by qPCR in the indicated lines. c Western shows effects of p27CK-DD on total and activated STAT3 and Pyk2 and effects of PYK2 loss on STAT3 levels and STAT3pY705. d Western shows effects of p27pT198 (reduced by PI3K inhibitor, PF1502) and Pyk2 loss (siPYK2) and Pyk2 inhibition by PF431396 on STAT3 levels and activation (STAT3pY705) e p27, STAT3, and cJun co-occupied peaks at +2 kb of the PTPN12 TSS. f–k ChIP-qPCR shows p27 (f); STAT3 (g); cJun (h); SIN3A (i); YY1 (j) and HDAC1 (k) binding at +2 kb of the PTPN12 TSS where p27 peaks overlap with binding peaks of STAT3 and cJun. l cJun ChIP-Western blot (right) shows interaction on chromatin of the indicated proteins. Input is shown on the left. m Schematic representation for p27/STAT3/cJun in activator (left) and repressor (right) complexes. All graphs show mean ± SEM from N = 3 biological replicate assays. p-values were calculated by paired one-tailed Student’s T Test and are indicated in graphs. Source data are provided as a Source data file. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41467-024-48742-y), licensed under a CC-BY license. Not internally tested by R&D Systems.