Human Phospho-SYK (Y525/Y526) Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Phospho-SYK (Y525/Y526) by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 10 µg/mL IgM for 2 minutes and U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with 2nM pervanadate (PV) for 5 minutes. PVDF Membrane was probed with 0.1 µg/mL of Human Phospho-SYK (Y525/Y526) Monoclonal Antibody (Catalog # MAB6459) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phopho-SYK (Y525/Y526) at approximately 80 kDa (as indicated). For additional reference, the membrane was stripped and reprobed with mouse anti-SYK (lower panel). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SYK
Spleen tyrosine kinase (SYK; also tyrosine-protein kinase SYK) is a 70-80 kDa, ubiquitously expressed member of the protein kinase superfamily, the tyrosine protein kinase family, and the SYK/ZAP-70 subfamily of proteins. Human SYK is 635 amino acids (aa) in length and contains two SH2 domains (aa 14-106 and aa 168-259), and one protein kinase domain (aa 371-631). A splicing variant produces a second isoform that has a deletion of aa 371-631 found in the longer isoform. Human SYK shares 92% and 91% aa sequence identity with mouse and rat SYK, respectively. Functionally, SYK is a positive effector of B-cell antigen receptor-stimulated responses.
Product Datasheets
Citations for Human Phospho-SYK (Y525/Y526) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Heightened Procoagulation after Post-Operative Thromboprophylaxis Completion in Patients with Metastatic Bone Disease from Primary Colorectal Cancer
Authors: L Yamaura, D Young, L Skeith, MJ Monument, CN Jenne, A Dufour, P Schneider, EO Agbani
Journal of Clinical Medicine, 2022-12-13;11(24):.
Species: Human
Sample Types: Plasma
Applications: Western Blot -
SYK is activated by mutated MYD88 and drives pro-survival signaling in MYD88 driven B-cell lymphomas
Authors: M Munshi, X Liu, JG Chen, L Xu, N Tsakmaklis, MG Demos, A Kofides, ML Guerrera, C Jimenez, GG Chan, ZR Hunter, ML Palomba, KV Argyropoul, K Meid, A Keezer, J Gustine, T Dubeau, JJ Castillo, CJ Patterson, J Wang, SJ Buhrlage, NS Gray, SP Treon, G Yang
Blood Cancer J, 2020-01-31;10(1):12.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot -
Distinct Spatiotemporal Distribution of Bacterial Toxin-Produced Cellular cAMP Differentially Inhibits Opsonophagocytic Signaling
Authors: S Hasan, WU Rahman, P Sebo, R Osicka
Toxins (Basel), 2019-06-20;11(6):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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