Human Phospho-Vav-1 (Y160) Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB37861V Datasheet / COA / SDS

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FAB37861V-100UG

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Human Phospho-Vav-1 (Y160) Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human VAV-1 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG2a Clone # 751411

Purification

Protein A or G purified

Immunogen

Phosphopeptide containing the human Vav-1 Y160 site

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Vav-1

The Vav-1 proto-oncogene is a 95 kDa guanine nucleotide exchange factor (GEF) for the low molecular weight GTPases of the Rho/Rac family. Vav-1 is a cytosolic protein that is primarily expressed in hematopoietic cells and plays an essential role in the proliferation and activation of T and B cells. It promotes intracellular signaling by its multiple protein binding motifs. Vav-1 is phosphorylated on Y160 after cell adhesion via integrin avb3. This phosphorylation is necessary for adhesion‑mediated Rho activation and association between avb3 and Vav-1. The peptide immunogen represents a sequence that is conserved between human, mouse and rat Vav-1.

Entrez Gene IDs

7409 (Human); 22324 (Mouse); 25156 (Rat)

Alternate Names

oncogene vav; proto-oncogene vav; vav 1 guanine nucleotide exchange factor; vav proto-oncogene; Vav1; Vav-1; vav-T; VAVvav 1 oncogene