Human Plasminogen Antibody (270412)

R&D Systems | Catalog # MAB2596

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 270412
View all Plasminogen Primary Antibodies »

Product Specifications

Immunogen

Human plasma-derived Plasminogen

Specificity

Detects human Plasminogen and Plasmin in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Plasminogen Antibody (270412)

Detection of Plasminogen/Plasmin by Western Blot

Detection of Plasminogen/Plasmin by Western Blot

Binding of Lec‐bs IgG to purified fibrinogen. (A) WB using Lec‐bs IgG on samples of purified plasminogen, purified fibrinogen, and donor's plasma. Lec‐bs IgG‐immunoreactive bands are observed only in lanes containing purified fibrinogen and donor plasma. (B) Sandwich ELISA for purified human fibrinogen and plasminogen using lecanemab as a capture antibody and anti‐human fibrinogen antibody as the detection antibody coupled with an anti‐mouse IgG peroxidase‐labeled antibody. A dynamic response is observed with purified fibrinogen, which is statistically significant and different from the control (purified plasminogen) at any concentration (P < 0.0001). (C) Graph showing disequilibrium ratios of biotinylated Lec‐bs IgG between C1 and C2 after 12 h of incubation (as in Fig. 1) when IgGs were spiked into PBS (n = 3) or in a purified fibrinogen solution (n = 6). Error bars, 95% CI; dotted lines, median value. Nonsignificantly different from equilibrium (ns); significantly different from equilibrium (*P = 0.0312). (D) Determination of KD of lecanemab for A beta 2‐42 peptide using SPR. (E) Determination of KD of lecanemab for human fibrinogen using SPR. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39476320), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Plasminogen Antibody (270412)

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Human Plasminogen (Catalog # 1939-SE), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Human Plasminogen (Catalog # 1939-SE) under non-reducing conditions only

Reviewed Applications

Read 1 review rated 4 using MAB2596 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Plasminogen

Plasminogen (PLG) is the precursor of Plasmin, an active serine protease that dissolves the fibrin of blood clots and acts in many other processes such as embryonic development, tissue remodeling, inflammation and tumor invasion (1, 2). Synthesized in the kidney, Plasminogen is found in plasma and many extracellular fluids. Activated by u- or t-Plasminogen Activator, the single‑chain Plasminogen (aa 20-810) is converted to Plasmin, which consists of disulfide bond‑linked heavy chain A (aa 20-580) and light chain B (aa 581-810). Heavy chain A contains 5 kringle domains and light chain B corresponds to the serine protease domain. A fragment consisting of the first 4 kringle domains has been named Angiostatin, an angiogenesis inhibitor (3, 4).

References

  1. Petersen, T.E. et al. (1990) J. Biol. Chem. 265:6104.
  2. Forsgren, M. et al. (1987) FEBS Lett. 213:254.
  3. O’Reilly, M.S. et al. (1994) Cell 79:315.
  4. Sim, B.K. et al. (1997) Cancer Res. 57:1329.

Alternate Names

Plg

Entrez Gene IDs

5340 (Human); 18815 (Mouse); 85253 (Rat)

Gene Symbol

PLG

Additional Plasminogen Products

Product Documents for Human Plasminogen Antibody (270412)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Select SDS Language

Can't find your language? Request a SDS in your language here.

Product Specific Notices for Human Plasminogen Antibody (270412)

For research use only

Citations for Human Plasminogen Antibody (270412)

Customer Reviews for Human Plasminogen Antibody (270412) (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human Plasminogen Antibody (270412)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 的 1 review Showing All
Filter By:
Clear Filters X
  • Human Plasminogen/Plasmin Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/13/2019
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human Plasminogen Antibody (270412) MAB2596

There are no reviews that match your criteria.

Showing  1 - 1 的 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies