Human Polypeptide GalNAc Transferase 3/GALNT3 Antibody

Detection of Human and Mouse Polypeptide GalNac Transferase 3/GALNT3 by Western Blot.

Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and mouse testis tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Polypeptide GalNac Transferase 3/GALNT3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7174) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Polypeptide GalNac Transferase 3/GALNT3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Polypeptide GalNac Transferase 3/GALNT3 in HeLa Human Cell Line.

Polypeptide GalNac Transferase 3/GALNT3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Polypeptide GalNac Transferase 3/GALNT3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7174) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Golgi granules. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human Polypeptide GalNAc Transferase 3/GALNT3 by Western Blot

GALNT3 is overexpressed in Fucosylated cell lines and contributes to its glyco-phenotype.a Volcano plot of a differential gene expressed genes between the Lewishigh and Lewislow cell lines defined in. b Scheme of the GALNT3 enzymatic activity. c Expression of GALNT3 in the different tumour clusters in scRNA-seq. d Expression of Tn antigen (mAb: 83D4) and Sialyl-Tn antigen (mAb: B72.3) was analyzed by flow cytometry. e Western blot of GALNT3 in selected PDAC cell lines. f Confocal microscopy of GALNT3 (green) and actin (red). g Expression of GALNT3 is associated with the Fucosylated subtype in PDAC tissue. h Analysis of the expression of glycan structures using plant lectins and antibodies by flow cytometry in WT and GALNT3 KO cell lines. Statistical analysis: Two-way ANOVA with Sidak method for multiple test correction (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001). i Western Blot of GALNT3, E-Cadherin and Actin in WT and GALNT3 KO cell lines. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35017635), licensed under a CC-BY license. Not internally tested by R&D Systems.