Human PP1 alpha Catalytic Subunit Antibody (490314)

Detection of Human PP1 alpha by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, CHP-100 human neuroblastoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human PP1 alpha Catalytic Subunit Monoclonal Antibody (Catalog # MAB6105) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human PP1 alpha, recombinant human PP1 beta, and recombinant human PP1 gamma (5 ng/lane) were included. A specific band was detected for PP1 apha at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Detection of Human PP1 alpha/PPP1A by Western Blot

LSP1 and supervillin interact with a similar subset of myosin IIA regulators. a Western blots of macrophage lysates progressively extracted by increased concentration of TritonX-100 (1–5%), or in RIPA buffer. Note that LSP1, and also myosin IIA, are mostly extracted in the 2% and RIPA fractions. b Western blot of immunoprecipitation of endogenous LSP1 or myosin IIA from macrophage lysates, with control IgG and input. Blots were probed with indicated antibodies. Note cross-coprecipitation of LSP1 and myosin IIA, accompanied by coprecipitation of myosin regulators MLCK and calmodulin. Molecular weight in kDa is indicated. c Western blots of anti-GFP immunoprecipitation of lysates from macrophages expressing LSP1 full length or LSP1 domains fused to GFP (For inputs, see Supplementary Fig. 7A). Blots were probed with indicated antibodies. Note coprecipitation of myosin IIA with full length LSP1, and also with the C-terminal and the villin headpiece-like domains (V1V2) of LSP1. Interestingly only the full length and the C-terminal constructs, but not V1V2, are also able to bind MLCK, pMLC and calmodulin. An LSP1-positive band in the lane of the C-terminal construct probably reflects the fact that the anti-LSP1 antibody recognizes an epitope in the C-terminal half of LSP1. d Immunoprecipitation of myosin IIA from macrophage lysates, with addition of Mg2+/ATP and/or latrunculin A (10 µM), as indicated. Upper panel: colloidal Coomassie stained SDS PAGE gel, lower panels: corresponding western blots developed with indicated antibodies. Molecular weight is indicated in kDa. e Quantification of coprecipitated amounts of actin and LSP1, normalized to precipitations performed without addition of Mg2+/ATP and/or latrunculin A. f Western blots of anti-GFP immunoprecipitations from lysates of macrophages expressing LSP1-GFP or supervillin construct SV1-830-GFP developed with indicated primary antibodies. Molecular weight in kDa is indicated. Note that LSP1 and SV1-830 coprecipitate

Detection of PP1 alpha by Western Blot

Detection of TIMAP–PP1c delta in SH-SY5Y cells. (A) RT-PCR was performed using specific primer pairs that targeted TIMAP, PP1c delta and GAPDH in BPAEC and SH-SY5Y cells. (B) Western blot analysis of BPAEC and SH-SY5Y cells using TIMAP- and PP1c delta -specific antibodies. Actin was utilized as a loading control. (C) TIMAP or PP1c delta was subjected to immunoprecipitation from lysates of SH-SY5Y cells. IP complexes were subjected to Western blot to detect the presence of TIMAP, PP1c delta and PP1c alpha. The negative control utilized was rabbit IgG. (D) Glutathione S transferase (GST) and GST-TIMAP were immobilized on glutathione Sepharose 4B beads, then the resin samples were incubated with SH-SY5Y cell lysate. Samples were tested for TIMAP, ERM, merlin and RACK1 by Western blot. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/24/24/17360), licensed under a CC-BY license. Not internally tested by R&D Systems.