Human PRDM14 Alexa Fluor® 594-conjugated Antibody

Catalog #: AF6175T Datasheet / COA / SDS

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AF6175T-100UG

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All PRDM14 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PRDM14 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human PRDM14 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PRDM14
Ala2-Asp201
Accession # Q9GZV8

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PRDM14

PRDM14 is an approximately 65 kDa transcriptional inhibitor that contains one histone methylating PR/SET domain (aa 266-367) and six zinc finger repeats (aa 400‑568). PRDM14 is preferentially expressed in germ cells and undifferentiated embryonic stem cells in which it plays a central role in lineage specification, maintenance of pluripotency, and repression of somatic gene transcription. PRDM14 is up‑regulated in breast cancer and lymphoblastic leukemia. Within aa 1-201, human and mouse PRDM14 share 46% aa sequence identity.

Long Name

PR Domain Containing 14

Entrez Gene IDs

63978 (Human); 383491 (Mouse)

Alternate Names

MGC59730; PFM11; PR domain containing 14; PR domain zinc finger protein 14; PR domain-containing protein 14; PRDM14; PR-domain zinc finger protein 14