Human Profilin 1 Antibody (816536)

R&D Systems | Catalog # MAB7779

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 816536
View all Profilin 1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Profilin 1
Met1-Tyr140
Accession # P07737

Specificity

Detects human Profilin 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Profilin 2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Profilin 1 Antibody (816536)

Detection of Human and Mouse Profilin 1 antibody by Western Blot.

Detection of Human and Mouse Profilin 1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Profilin 1 Monoclonal Antibody (Catalog # MAB7779) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Profilin 1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Profilin 1 by Simple WesternTM.

Simple Western shows lysates of Exosome Standards (LNCaP) (NBP3-11687) and Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Profilin 1 at approximately 17 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Profilin 1 Monoclonal Antibody (Catalog # MAB7779). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.

Applications for Human Profilin 1 Antibody (816536)

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: Exosome Standards (LNCaP) (Catalog# NBP3-11687) and Jurkat human acute T cell leukemia cell line

Western Blot

0.2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Profilin 1

Profilin 1 (gene name PFN1) is a ubiquitous, cytoplasmic, 140 amino acid, 12-15 kDa actin monomer-binding protein. It facilitates adenine nucleotide exchange, supplying ATP for actin filament polymerization. It is phosphorylated and acetylated, and binds proline-rich sequences. Human Profilin 1 shares 96% amino acid sequence identity with mouse and rat Profilin 1.

Alternate Names

PFN1, PROF1, Profilin I

Entrez Gene IDs

5216 (Human); 18643 (Mouse)

Gene Symbol

PFN1

UniProt

P07737 (Human)

Additional Profilin 1 Products

Product Documents for Human Profilin 1 Antibody (816536)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Profilin 1 Antibody (816536)

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Human Profilin 1 Antibody (816536)

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