Human Prohibitin Alexa Fluor® 750-conjugated Antibody

Catalog #: AF3470S Datasheet / COA / SDS

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AF3470S-100UG

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All Prohibitin Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Prohibitin Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Prohibitin in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Prohibitin
Ala2-Gln272
Accession # P35232

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Prohibitin

Prohibitin is an approximately 30 kDa protein that belongs to the prohibitin family. It contains a characteristic prohibitin domain and has been shown to have multiple functions. It can be localized to the mitochondria, nucleus, plasma membrane, and is also present in circulation. Post translational modifications of Prohibitin, including phosphorylation and ubiquitination, also occurs. Human Prohibitin shares 99% amino acid sequence identity with the mouse or rat protein.

Entrez Gene IDs

5245 (Human); 18673 (Mouse); 25344 (Rat)

Alternate Names

PHB; PHB1; Prohibitin