Human Rap2B Antibody (455517)

R&D Systems | Catalog # MAB5710

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 455517
View all Rap2B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Rap2B
Met1-Leu183
Accession # P61225

Specificity

Detects human Rap2B in Western blots. No cross-reactivity with recombinant human Rap2A is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Rap2B Antibody (455517)

Detection of Human Rap2B antibody by Western Blot.

Detection of Human Rap2B by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human Rap2B Monoclonal Antibody (Catalog # MAB5710) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Rap2A and Rap2B (5 ng/lane) were included. A specific band for Rap2B was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human Rap2B Antibody (455517)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Rap2B

Rap2B is a member of the small GTPase superfamily and ras family of proteins. Unlike normal ras proteins that have a nontransforming glutamine residue at aa 61, Rap2B has a threonine residue in that position. This change makes the intrinsic GTPase activity for Rap2B lower, thereby allowing it to exist in the activated state for a longer period of time than normal ras proteins. Aggregation of platelets by thrombin results in the activation of Rap2B in a Ca2+ and PI3 kinase-dependent manner.

Long Name

Ras-related Protein 2B

Alternate Names

MGC20484, RAP2B, member of RAS oncogene family, Ras family small GTP binding protein RAP2B, ras-related protein Rap-2b, small GTP binding protein

Entrez Gene IDs

5912 (Human); 74012 (Mouse); 170923 (Rat)

Gene Symbol

RAP2B

UniProt

P61225

Additional Rap2B Products

Product Documents for Human Rap2B Antibody (455517)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Rap2B Antibody (455517)

For research use only

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