Human Ras Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB3429U Datasheet / COA / SDS

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FAB3429U-100UG

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Human Ras Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Ras (H-Ras, N-Ras, and K-Ras) in Western blots. Does not detect more distantly related Ras superfamily members, including R-Ras, Ral, Rap, and Rheb.

Source

Monoclonal Rat IgG2b Clone # 342404

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human K-Ras2B
Met1-Met188 (Gln61His)
Accession # P01116-2

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Ras

The Ras proteins (H-Ras, N-Ras, and two splice variants of K-Ras) define a closely related family of 21 kDa GTPases that cycle between GTP-bound active and GDP-bound inactive states at the cytoplasmic face of the plasma membrane. Receptor tyrosine kinases and G-protein coupled receptors activate Ras, and effectors include Raf and PI 3-kinase. Activating mutations of Ras, which impair GTPase activity and stabilize the active state, are found in nearly one-third of all human cancers.

Long Name

v-Ki-ras2 Kirsten Rat Sarcoma Viral Oncogene Jomolog

Entrez Gene IDs

3845 (Human); 16653 (Mouse); 24525 (Rat)

Alternate Names

c-K-ras; GTPase HRas; GTPase NRas; Harvey rat sarcoma viral oncogene homolog; HRAS; HRAS1; Ki-Ras; KRAS; KRAS1; KRAS2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; N-ras; NRAS1; Oncogene Ras; proto-oncogene GTPase; Ras family small GTP binding protein H-Ras; Ras; RASK2