Human/Rat GOLGB1/Giantin Alexa Fluor® 532-conjugated Antibody

Catalog #: AF8159X Datasheet / COA / SDS

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AF8159X-100UG

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Human/Rat GOLGB1/Giantin Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human, Rat

Specificity

Detects human GOLGB1/Giantin in direct ELISAs. Detects human and rat GOLGB1/Giantin in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human GOLGB1/Giantin
Ser1596-Glu1702
Accession # Q14789

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: GOLGB1/Giantin

GOLGB1, also known as Giantin and Macrogolgin, is a 360 to 400 kDa member of the Golgin protein family. GOLGB1 is an integral component of the Golgi membrane that associates with the cytoskeleton. It may be involved in both forming intercisternal cross-bridges that mediate stacking in Golgi complexes, and in reorganizing the complexes after they fragment during mitosis. GOLGB1 has also been identified as an autoantigen in chronic rheumatoid arthritis, and in the autoimmune disease Sjoegren syndrome. Human GOLGB1 is 3259 amino acids (aa) in length, and over aa 1596-1702, shares 92% and 91% sequence identity with its mouse and rat orthologs, respectively.

Long Name

Golgin B1

Entrez Gene IDs

2804 (Human); 224139 (Mouse); 192243 (Rat)

Alternate Names

372 kDa Golgi complex-associated protein; GCP; GCP372; GCP372GIANTIN; Giantin; GOLGB1; golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal)1,macrogolgin; golgi integral membrane protein 1; golgin B1; golgin B1, golgi integral membrane protein; Golgin subfamily B member 1; GOLIM1; Macrogolgin